三维与二维培养的直接比较揭示了老年成骨细胞在三维培养中具有更高的成骨能力。
Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D.
机构信息
Department of Experimental Trauma Surgery, Klinikum Rechts der Isar, Technical University Munich, Munich, Germany.
Department of Orthopedics and Trauma Surgery, Division of Trauma Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
出版信息
J Orthop Surg Res. 2021 Jan 6;16(1):13. doi: 10.1186/s13018-020-02153-z.
BACKGROUND
The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture.
METHODS
Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression.
RESULTS
Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002).
CONCLUSION
Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people.
TRIAL REGISTRATION
5217/11 on the 22nd of Dec. 2011.
背景
本研究旨在探讨供体年龄较大的人源成骨细胞在 2D 和 3D 培养条件下的成骨潜能。
方法
使用相同的聚苯乙烯材料，在 2D 和 3D 培养条件下诱导成骨细胞向成骨细胞分化并进行培养，培养时间为 2 周。取样本评估碱性磷酸酶（ALP）活性、矿化和基因表达。
结果
与 0 天（p<0.0001）和 2D（p<0.0001）相比，3D 培养第 7 天 OPG 水平显著升高（8.2 倍）。两种培养体系的骨钙素（OC）水平均降低（2D 为基础值的 85%，3D 为 50%）。第 7 天 3D 培养的胶原 1（Col1）表达升高（1.4 倍；p=0.009）。第 7 天 3D 培养的骨桥蛋白（OP）表达水平显著升高（6.5 倍；p=0.002）。第 14 天 3D 培养的矿化水平显著升高（p=0.0002）。
结论
与单层培养相比，供体年龄较大的人源原代成骨细胞在 3D 培养条件下 OPG、Col1 和 OP 的基因表达水平显著升高。因此，老年人成骨细胞中可能仍然存在相对较高的天然 3D 排列成骨潜力。
试验注册号
5217/11 于 2011 年 12 月 22 日注册。
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