Department of Pediatrics, Penn State College of Medicine, Hershey, PA, USA.
Department of Public Health Sciences, Penn State College of Medicine, Hershey, PA, USA.
J Dev Orig Health Dis. 2021 Dec;12(6):876-882. doi: 10.1017/S2040174420001324. Epub 2021 Jan 7.
Rapid infant growth increases the risk for adult obesity. The gut microbiome is associated with early weight status; however, no study has examined how interactions between microbial and host ribonucleic acid (RNA) expression influence infant growth. We hypothesized that dynamics in infant stool micro-ribonucleic acids (miRNAs) would be associated with both microbial activity and infant growth via putative metabolic targets. Stool was collected twice from 30 full-term infants, at 1 month and again between 6 and 12 months. Stool RNA were measured with high-throughput sequencing and aligned to human and microbial databases. Infant growth was measured by weight-for-length z-score at birth and 12 months. Increased RNA transcriptional activity of Clostridia (R = 0.55; Adj p = 3.7E-2) and Burkholderia (R = -0.820, Adj p = 2.62E-3) were associated with infant growth. Of the 25 human RNAs associated with growth, 16 were miRNAs. The miRNAs demonstrated significant target enrichment (Adj p < 0.05) for four metabolic pathways. There were four associations between growth-related miRNAs and growth-related phyla. We have shown that longitudinal trends in gut microbiota activity and human miRNA levels are associated with infant growth and the metabolic targets of miRNAs suggest these molecules may regulate the biosynthetic landscape of the gut and influence microbial activity.
婴儿快速生长会增加成年后肥胖的风险。肠道微生物群与早期体重状况有关；然而，尚无研究探讨微生物和宿主核糖核酸（RNA）表达之间的相互作用如何影响婴儿的生长。我们假设婴儿粪便中小核糖核酸（miRNA）的动态变化将通过潜在的代谢靶标与微生物活性和婴儿生长相关。从 30 名足月婴儿中收集粪便，在 1 个月和 6 至 12 个月之间各收集两次。使用高通量测序测量粪便 RNA，并与人类和微生物数据库进行比对。通过出生时和 12 个月时的体重-身长 z 评分来测量婴儿的生长情况。梭菌（R = 0.55；调整后的 p 值 = 3.7E-2）和伯克霍尔德菌（R = -0.820，调整后的 p 值 = 2.62E-3）的 RNA 转录活性增加与婴儿生长有关。在与生长相关的 25 个人类 RNA 中，有 16 个是 miRNA。这些 miRNA 显示出对四个代谢途径的显著靶标富集（调整后的 p 值 < 0.05）。在与生长相关的 miRNA 和与生长相关的菌门之间有四个关联。我们已经表明，肠道微生物群活性和人类 miRNA 水平的纵向趋势与婴儿生长有关，并且 miRNA 的代谢靶标表明这些分子可能调节肠道的生物合成景观并影响微生物的活性。
