Alemu Belete Kassa, Misganaw Desye, Mengistu Getnet
Pharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
Clin Pharmacol. 2020 Dec 31;12:213-222. doi: 10.2147/CPAA.S288394. eCollection 2020.
is traditionally used for the treatment of wounds and various bacterial infections. Due to the ongoing escalation of antimicrobial resistance, there is an increasing demand for the appropriate wound care and hence, the present study was initiated to investigate the wound healing effects of the leaf extract ointments of in mice and its in-vitro antioxidant activity.
The crude extract was prepared as 5% and 10% w/w ointments for topical use in mice. Wound contraction and epithelialization period were determined in excision and infected models, whereas tensile strength was determined in an incision model. Besides, its antioxidant activity was evaluated using the DPPH method.
In this study, the 10% w/w extract ointment did not cause toxicity at the 2000 mg/kg limit dose. In the excision model, the 10% w/w ointment exhibited a significant wound contraction effect starting from day 6 to 14 with a complete epithelization shown on day 13. Besides, the 5%w/w ointment showed a significant wound contraction effect starting from day 6 onwards, and a significant decrease in the epithelization period observed on day 16. Conversely, both the 10% w/w and 5% w/w ointments showed significant wound contraction effects starting from day 4 and onwards in the infected model. However, a complete epithelization period was observed on days 14 and 18 in the 10%w/w and 5% w/w/extract ointment treated groups, respectively. In the incision model, the 10% (w/w) and 5% (w/w) extract ointments showed a significant increase in tensile strength by 36.80 and 32.23%, respectively. Moreover, the antioxidant activity of the extract was concentration-dependent with an IC50 value of 5.49± 0.38 µg/µL.
The potential wound healing effects of this plant may provide a candidate source in the discovery of new drugs for the treatment of wounds.
传统上用于治疗伤口和各种细菌感染。由于抗菌药物耐药性的不断升级，对适当的伤口护理的需求日益增加，因此，开展了本研究以调查[植物名称]叶提取物软膏对小鼠的伤口愈合作用及其体外抗氧化活性。
将粗提取物制成5%和10%（w/w）的软膏用于小鼠局部使用。在切除和感染模型中测定伤口收缩和上皮化时间，而在切口模型中测定抗张强度。此外，使用DPPH方法评估其抗氧化活性。
在本研究中，10%（w/w）提取物软膏在2000mg/kg极限剂量下未引起毒性。在切除模型中，10%（w/w）软膏从第6天到第14天表现出显著的伤口收缩作用，第13天显示完全上皮化。此外，5%（w/w）软膏从第6天起表现出显著的伤口收缩作用，第16天观察到上皮化时间显著缩短。相反，在感染模型中，10%（w/w）和5%（w/w）软膏从第4天起均表现出显著的伤口收缩作用。然而，在10%（w/w）和5%（w/w）提取物软膏治疗组中，分别在第14天和第18天观察到完全上皮化。在切口模型中，10%（w/w）和5%（w/w）提取物软膏的抗张强度分别显著增加了36.80%和32.23%。此外，提取物的抗氧化活性呈浓度依赖性，IC50值为5.49±0.38μg/μL。
这种植物潜在的伤口愈合作用可能为发现治疗伤口的新药提供候选来源。
