（布鲁斯）J.F. 格梅尔80%甲醇粗花提取物对小鼠伤口愈合和抗炎活性的体内评价
In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of (Bruce) J.F. Gmel in Mice.
作者信息
Belachew Teshome Fentik, Asrade Seyfe, Geta Mestayt, Fentahun Engidaw
机构信息
Department of Pharmacy, Debre Birhan Health Science College, Debre Birhan, Ethiopia.
Department of Pharmacology, School of Pharmacy, University of Gondar, Gondar, Ethiopia.
出版信息
Evid Based Complement Alternat Med. 2020 Mar 30;2020:9645792. doi: 10.1155/2020/9645792. eCollection 2020.
BACKGROUND
(Bruce) J.F. Gmel (Rosaceae) is distributed in the highlands of Ethiopia. The flowers of (Bruce) J.F. Gmel are used traditionally to treat wound. However, there was no scientific report on wound healing activity of (Bruce) J.F. Gmel. Thus, this study was initiated to investigate the wound healing and anti-inflammatory activities of 80% methanol crude extract of flowers of in mice.
OBJECTIVE
The objective of this study was to evaluate the wound healing and anti-inflammatory activity of 80% methanol crude flower extract of in mice.
METHODS
Air-dried flowers of were grounded and macerated three times successively by 80% methanol. The dried extract was fractionated with chloroform, ethyl acetate, and water. Phytochemical screening tests were performed according to established procedures. The crude extract and solvent fractions were formulated as ointments. Wound healing activity of the crude extract was evaluated using excision and incision wound models, and the wound healing activities of solvent fractions were evaluated by using the excision wound model. The anti-inflammatory activity of the 80% methanol extract of was evaluated using carrageenan-induced hind paw edema model in mice.
RESULT
The 2000 mg/kg test dose of the 10% (w/w) crude extract ointment was safe in rats. Both the 5% (w/w) and 10% (w/w) crude extract ointment-treated groups showed significant wound contraction starting from the day 4. Both 5% (w/w) and 10% (w/w) crude extract ointments showed significant ( < 0.001) increment of tensile strength compared to the negative control. The 10% (w/w) aqueous and ethyl acetate fraction ointment revealed high ( < 0.001) percentage of wound contraction. The 100 mg/kg, 200 mg/kg, and 400 mg/kg oral administration of the crude extract had significant inhibition of the paw edema in mice of carrageenan-induced inflammation.
CONCLUSION
The results of this study evidenced that both 5% w/w and 10% w/w 80% methanol extract ointment of the flowers of have wound healing and anti-inflammatory effects.
背景
（布鲁斯）J.F. 格梅尔（蔷薇科）分布于埃塞俄比亚高地。传统上，（布鲁斯）J.F. 格梅尔的花用于治疗伤口。然而，关于（布鲁斯）J.F. 格梅尔伤口愈合活性尚无科学报道。因此，本研究旨在调查（布鲁斯）J.F. 格梅尔花80%甲醇粗提物在小鼠中的伤口愈合和抗炎活性。
目的
本研究的目的是评估（布鲁斯）J.F. 格梅尔花80%甲醇粗提物在小鼠中的伤口愈合和抗炎活性。
方法
将（布鲁斯）J.F. 格梅尔的风干花研磨，并先后用80%甲醇浸渍三次。干燥提取物用氯仿、乙酸乙酯和水进行分离。按照既定程序进行植物化学筛选试验。将粗提物和溶剂提取物配制成软膏。使用切除和切开伤口模型评估粗提物的伤口愈合活性，使用切除伤口模型评估溶剂提取物的伤口愈合活性。使用角叉菜胶诱导的小鼠后爪水肿模型评估（布鲁斯）J.F. 格梅尔80%甲醇提取物的抗炎活性。
结果
10%（w/w）粗提物软膏2000mg/kg的试验剂量对大鼠是安全的。5%（w/w）和10%（w/w）粗提物软膏处理组从第4天开始均显示出显著的伤口收缩。与阴性对照相比，5%（w/w）和10%（w/w）粗提物软膏的抗张强度均有显著（<0.001）增加。10%（w/w）水相和乙酸乙酯相提取物软膏显示出高（<0.001）的伤口收缩百分比。粗提物100mg/kg、200mg/kg和400mg/kg口服给药对角叉菜胶诱导炎症的小鼠爪水肿有显著抑制作用。
结论
本研究结果证明，（布鲁斯）J.F. 格梅尔花的5%（w/w）和10%（w/w）80%甲醇提取物软膏具有伤口愈合和抗炎作用。
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