Copete Elena, Copete Miguel A, Ferrandis Pablo, Herranz José M
E.T.S.I.A.M. Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, Albacete, Spain.
Institute of Botany, University of Castilla-La Mancha, Albacete, Spain.
AoB Plants. 2020 Nov 17;12(6):plaa060. doi: 10.1093/aobpla/plaa060. eCollection 2020 Dec.
Seed dormancy classes determine both population and species-level processes which can be crucial in the life cycle of many plants. However, there are no studies of a dormancy cline between levels of morphophysiological dormancy (MPD). We aimed to determine the class of seed dormancy of exhibits in order to explore links between different dormancy levels, previously characterized in two closely related phylogenetic congeners, and . Experiments were carried out under both near-natural temperature and controlled laboratory conditions. The parameters calculated were mean embryo length, radicle and shoot emergence percentages. The effects of different periods of storage; and different periods with or without GA of warm, cold or warm plus cold were analysed. The populations from the Baetic System of mountain ranges in south-eastern Spain show clinal variation in a northeast-southwest gradient from intermediate to non-deep complex MPD, through the coexistence of intermediate and non-deep complex MPD in (21 % and 74 %, respectively). In addition, 54 % of stored seeds were able to show both levels of MPD. occupies an intermediate position between and in the geographical distribution and in the clinal germination ranges. It strongly suggests an evolutionary gradient, which connects the intermediate complex MPD with the non-deep complex MPD in southern Iberian daffodils. This is the first study showing a gradient in the evolution between levels of MPD. Our results demonstrate a cline in these levels in response to both an environmental gradient and genetic differences.
种子休眠类别决定了种群和物种水平的过程，这在许多植物的生命周期中可能至关重要。然而，目前尚无关于形态生理休眠（MPD）水平之间休眠渐变群的研究。我们旨在确定[具体植物名称]种子的休眠类别，以探索不同休眠水平之间的联系，此前已在两个密切相关的系统发育同属植物[具体植物名称1]和[具体植物名称2]中对这些休眠水平进行了表征。实验在接近自然温度和受控实验室条件下进行。计算的参数包括平均胚长度、胚根和芽出现百分比。分析了不同储存期以及不同时期使用或不使用赤霉素（GA）、温暖、寒冷或温暖加寒冷处理的影响。来自西班牙东南部山脉贝蒂克系统的[具体植物名称]种群呈现出东北 - 西南梯度的渐变变异，从中间型到非深度复杂MPD，通过[具体植物名称3]中中间型和非深度复杂MPD的共存（分别为21%和74%）。此外，54%的储存种子能够表现出两种MPD水平。[具体植物名称]在地理分布和渐变发芽范围上处于[具体植物名称1]和[具体植物名称2]之间的中间位置。这强烈表明存在一种进化梯度，将伊比利亚南部水仙花的中间复杂MPD与非深度复杂MPD联系起来。这是第一项显示MPD水平之间进化梯度的研究。我们的结果表明，这些水平响应环境梯度和遗传差异而呈现出渐变群。
