ETSIAM, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, 02071 Albacete, Spain.
Botanical Institute, University of Castilla-La Mancha, Avenida de la Mancha s/n, 02006 Albacete, Spain.
Molecules. 2020 Sep 27;25(19):4439. doi: 10.3390/molecules25194439.
The daffodil L. contains alkaloids of pharmaceutical interest. Wild daffodil populations have diverse genetic backgrounds and various genetic traits of possible importance. Developing protocols for plant production from seeds may ensure the availability of a large reservoir of individuals as well as being important for species with bulbs that are difficult to acquire. The closely related subsp. and subsp. were investigated in this study because the alkaloids isolated from both are of high pharmacological interest. At the dispersal time, the seeds of both were dormant with underdeveloped embryos, i.e., morphophysiological dormancy (MPD). Experiments were conducted outdoors and under controlled laboratory conditions. Embryo growth and the percentages of radicle and seedling emergence were calculated under different temperature-light stratifications. In , embryo growth occurred during warm stratification (28/14 °C or 25/10 °C) and the radicle then emerged when the temperature decreased, but the shoot was dormant. In , the seeds germinated when cold stratified (5 °C) and then incubated at cool temperatures. Thus, and exhibit different levels of MPD, i.e., deep simple epicotyl and intermediate complex, respectively. Plant production protocols from seeds were established for both taxa in this study.
黄水仙 L. 含有具有药用价值的生物碱。野生黄水仙种群具有多样化的遗传背景和各种可能重要的遗传特征。制定从种子中进行植物生产的方案可以确保大量个体的可用性，并且对于那些球茎难以获取的物种也很重要。本研究调查了密切相关的亚种和亚种，因为从这两个亚种中分离出的生物碱都具有很高的药理学价值。在传播时间，这两个亚种的种子都处于休眠状态，胚未发育成熟，即形态生理休眠（MPD）。在户外和实验室控制条件下进行了实验。在不同的温度-光照分层条件下计算了胚的生长和胚根和幼苗出现的百分比。在亚种中，胚在温暖分层（28/14°C 或 25/10°C）期间生长，然后当温度下降时胚根出现，但芽休眠。在亚种中，种子在冷分层（5°C）时发芽，然后在凉爽的温度下培养。因此，亚种和亚种分别表现出不同程度的 MPD，即深简单上胚轴和中间复杂。本研究为这两个分类单元建立了从种子中进行植物生产的方案。
