Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China; Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410125, China.
Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China.
Res Vet Sci. 2021 Mar;135:8-14. doi: 10.1016/j.rvsc.2020.12.021. Epub 2020 Dec 29.
For a long time, phytogenic resources have been widely used as substitutes for antibiotics in livestock production. However, few studies have examined the relationship between the intestinal microbiota and fermented herbal medicines. Here, 252 Arbor Acres broiler plus broiler chickens were randomly assigned to a control group, which was fed a basal diet; an unfermented healthy chicken powder group (JJS) fed a basal diet containing 20 g/kg JJS; or one of 5 fermented JJS groups, which were fed a basal diet containing 20 g/kg JJS, fermented with by 5 different bacterial strains for 42 days. The growth performances of the different groups were measured and the changes in the intestinal microbiota were analyzed. The body weight gain in the Bacillus subtilis group (Bs) was the highest among the 6 groups, while the feed conversion ratio (FCR) was best with Z. rouxii fermentation. The result indicated that products of JJS fermentation products of JJS by B. subtilis and Z. rouxii had important effects on chicken growth performance. The foregut and hindgut microbial communities of Bs, Zr, the control group and the JJS group, were collected for 16S rDNA sequencing. The results showed that JJS and its fermentation products mainly acted on the foregut and had little effect on the hindgut, and Z. rouxii fermentation products can increased the diversity in the foregut microbial community. In addition, the relative abundance of Bifidobacterium in the foregut of the Z. rouxii group was significantly increased, which may be an important factor in promoting growth.
长期以来，植物源资源一直被广泛用作畜牧业抗生素的替代品。然而，很少有研究探讨肠道微生物群与发酵草药之间的关系。在这里，252 只 Arbor Acres 肉鸡和肉鸡被随机分配到对照组，喂食基础日粮；未发酵的健康鸡粉组（JJS）喂食含有 20g/kg JJS 的基础日粮；或 5 个发酵 JJS 组中的一个，喂食含有 20g/kg JJS 的基础日粮，用 5 种不同的细菌菌株发酵 42 天。测量了不同组的生长性能并分析了肠道微生物群的变化。枯草芽孢杆菌组（Bs）的体重增加最高，而发酵 Z. rouxii 的饲料转化率（FCR）最好。结果表明，JJS 发酵产品中枯草芽孢杆菌和 Z. rouxii 的产物对鸡的生长性能有重要影响。收集 Bs、Zr、对照组和 JJS 组的前肠和后肠微生物群落进行 16S rDNA 测序。结果表明，JJS 及其发酵产物主要作用于前肠，对后肠影响较小，而发酵产物可以增加前肠微生物群落的多样性。此外，发酵 Z. rouxii 组前肠双歧杆菌的相对丰度显著增加，这可能是促进生长的重要因素。
