干细胞领域中的单细胞测序
Single-cell Sequencing in the Field of Stem Cells.
作者信息
Chen Tian, Li Jiawei, Jia Yichen, Wang Jiyan, Sang Ruirui, Zhang Yi, Rong Ruiming
机构信息
1Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, P.R. China; 2Shanghai Key Laboratory of Organ Transplantation, Shanghai, P.R. China; 3Biomedical Research Center, Institute for Clinical Sciences, Zhongshan Hospital, Fudan University, Shanghai, P.R. China; 4Department of Urology, Shanghai Public Health Clinical Center, Shanghai, P.R. China; 5Department of Transfusion, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
出版信息
Curr Genomics. 2020 Dec;21(8):576-584. doi: 10.2174/1389202921999200624154445.
Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional sequencing analysis methods often cover up this difference. Single-cell sequencing technology refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It can effectively analyze cell heterogeneity and identify a small number of cell populations. With the continuous progress of cell sorting, nucleic acid extraction and other technologies, single-cell sequencing technology has also made great progress. Encouraging new discoveries have been made in stem cell research, including pluripotent stem cells, tissue-specific stem cells and cancer stem cells. In this review, we discuss the latest progress and future prospects of single-cell sequencing technology in the field of stem cells.
细胞间的变异和异质性是干细胞的基本特征。传统的测序分析方法常常掩盖了这种差异。单细胞测序技术是指在单细胞水平上对基因组进行高通量测序分析的技术。它能够有效地分析细胞异质性并识别少量细胞群体。随着细胞分选、核酸提取等技术的不断进步，单细胞测序技术也取得了巨大进展。在干细胞研究领域，包括多能干细胞、组织特异性干细胞和癌症干细胞等方面都有了令人鼓舞的新发现。在这篇综述中，我们讨论了单细胞测序技术在干细胞领域的最新进展和未来前景。
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