Hu Yue, Zhang Ying, Liu Yutong, Gao Yan, San Tiantian, Li Xiaoying, Song Sensen, Yan Binglong, Zhao Zhuo
Department of Cardiology, Jinan Central Hospital, Shandong University, Jinan, China.
Department of Cardiology, Central Hospital Affiliated Shandong First Medical University, Jinan, China.
Front Cardiovasc Med. 2022 Jul 26;9:905151. doi: 10.3389/fcvm.2022.905151. eCollection 2022.
Single-cell RNA sequencing (scRNA-seq) provides high-resolution information on transcriptomic changes at the single-cell level, which is of great significance for distinguishing cell subtypes, identifying stem cell differentiation processes, and identifying targets for disease treatment. In recent years, emerging single-cell RNA sequencing technologies have been used to make breakthroughs regarding decoding developmental trajectories, phenotypic transitions, and cellular interactions in the cardiovascular system, providing new insights into cardiovascular disease. This paper reviews the technical processes of single-cell RNA sequencing and the latest progress based on single-cell RNA sequencing in the field of cardiovascular system research, compares single-cell RNA sequencing with other single-cell technologies, and summarizes the extended applications and advantages and disadvantages of single-cell RNA sequencing. Finally, the prospects for applying single-cell RNA sequencing in the field of cardiovascular research are discussed.
单细胞RNA测序（scRNA-seq）在单细胞水平上提供了关于转录组变化的高分辨率信息，这对于区分细胞亚型、识别干细胞分化过程以及确定疾病治疗靶点具有重要意义。近年来，新兴的单细胞RNA测序技术已用于在解码心血管系统中的发育轨迹、表型转变和细胞相互作用方面取得突破，为心血管疾病提供了新的见解。本文综述了单细胞RNA测序的技术流程以及基于单细胞RNA测序在心血管系统研究领域的最新进展，将单细胞RNA测序与其他单细胞技术进行了比较，并总结了单细胞RNA测序的扩展应用及其优缺点。最后，讨论了单细胞RNA测序在心血管研究领域的应用前景。
