Department of Food Science & Biotechnology, College of Science and Engineering, Kyonggi University, 154-42, Gwanggyosan-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16227, Republic of Korea.
Department of Physicology, College of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Biomed Res Int. 2020 Dec 12;2020:3891806. doi: 10.1155/2020/3891806. eCollection 2020.
Berberine (BBR), a natural plant product, has been shown to have antidiabetic, cholesterol-reducing effects. To investigate the action of BBR as appetite suppressants, two experimental protocols were performed. In the first experiment, the mice were fed either a normal-chow diet or a high-fat diet (HF). The mice received daily intraperitoneal injections of BBR (10 mg/kg or saline at 1 ml/kg) for 3 weeks. To determine the antiobesity effects of BBR, the food consumption, body weight, fat contents, serum leptin, and glucose level were investigated. In the second experiment, we set out to validate the effect of BBR on central neuropeptide Y (NPY) stimulated rats. Experiments were carried out in 24-hour fasted rats, and then food intake and glucose level were subsequently recorded for 1 hour. The experimental groups were subdivided into the intra-3rd ventricular microinjections of ACSF (artificial cerebrospinal fluid), neuropeptide Y (NPY; 100 nM), NPY+BBR (10 nM), and NPY+BBR (100 nM) group. And then the blood glucose level was examined. In the first experiment, treatment with BBR in the HF diet mice reduced food intake, body weight, fat contents, serum leptin, and glucose level. In the second experiment, the NPY-injected group increased food intake by 39.3%, and food intake was reduced in the BBR group by 47.5%, compared with the ACSF-injected group. Also, the serum glucose level in the NPY+BBR (100 nM) group was significantly lower than that in the NPY (100 nM) group. The results suggest that BBR improved lipid dysregulation in obesity by controlling the central obesity related pathway.
小檗碱（BBR）是一种天然植物产物，已被证明具有降血糖和降低胆固醇的作用。为了研究 BBR 作为食欲抑制剂的作用，进行了两项实验方案。在第一项实验中，给小鼠喂食正常饮食或高脂肪饮食（HF）。小鼠每天接受腹腔内注射 BBR（10mg/kg 或 1ml/kg 的生理盐水），持续 3 周。为了确定 BBR 的抗肥胖作用，研究了食物消耗、体重、脂肪含量、血清瘦素和血糖水平。在第二项实验中，我们旨在验证 BBR 对中枢神经肽 Y（NPY）刺激大鼠的影响。实验在 24 小时禁食的大鼠中进行，然后记录 1 小时的食物摄入量和血糖水平。实验组分为侧脑室微量注射 ACSF（人工脑脊液）、NPY（100nM）、NPY+BBR（10nM）和 NPY+BBR（100nM）组。然后检查血糖水平。在第一项实验中，HF 饮食小鼠中 BBR 的治疗减少了食物摄入、体重、脂肪含量、血清瘦素和血糖水平。在第二项实验中，与 ACSF 注射组相比，NPY 注射组的食物摄入量增加了 39.3%，而 BBR 组的食物摄入量减少了 47.5%。此外，NPY+BBR（100nM）组的血清葡萄糖水平明显低于 NPY（100nM）组。结果表明，BBR 通过控制与中枢肥胖相关的途径改善了肥胖症中的脂质失调。
