Witherel Claire E, Gurevich David, Collin John D, Martin Paul, Spiller Kara L
School of Biomedical Engineering, Science, and Health Systems, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania 19104 United States.
Bristol Royal Infirmary, University Hospitals Bristol NHS Trust, Upper Maudlin Street, Bristol BS2 8HW, United Kingdom.
ACS Biomater Sci Eng. 2018 Apr 9;4(4):1233-1240. doi: 10.1021/acsbiomaterials.6b00760. Epub 2017 Apr 19.
Host-biomaterial interactions are critical determinants of the success or failure of an implant. However, detailed understanding of this process is limited due to a lack of dynamic tools for in vivo analyses. Here we characterize host-biomaterial interactions in zebrafish (), which are optically translucent and genetically tractable. Histological and immunohistochemical analyses following polypropylene suture implantation into adult zebrafish showed prolonged elevation of immune cell recruitment and collagen deposition, resembling a foreign body response. Live in vivo analysis showed that adsorption of the immunomodulatory cytokine interleukin-10 to a polystyrene microparticle, microinjected into transgenic larval zebrafish, inhibited neutrophil recruitment after 24 h compared to control microparticles, with no change in macrophage recruitment. This study illustrates that zebrafish are useful to investigate host-biomaterial interactions and have potential for high-throughput analysis of novel immunomodulatory biomaterials.
宿主与生物材料的相互作用是植入物成败的关键决定因素。然而，由于缺乏用于体内分析的动态工具，对这一过程的详细了解有限。在此，我们对斑马鱼体内的宿主与生物材料的相互作用进行了表征，斑马鱼具有光学透明性且易于进行基因操作。将聚丙烯缝线植入成年斑马鱼后进行的组织学和免疫组织化学分析显示，免疫细胞募集和胶原蛋白沉积持续升高，类似于异物反应。活体体内分析表明，将免疫调节细胞因子白细胞介素-10吸附到微注射到转基因斑马鱼幼体中的聚苯乙烯微粒上，与对照微粒相比，24小时后中性粒细胞募集受到抑制，而巨噬细胞募集没有变化。这项研究表明，斑马鱼有助于研究宿主与生物材料的相互作用，并且在新型免疫调节生物材料的高通量分析方面具有潜力。
