Bourdon Laura, Maurin Jean-Christophe, Gritsch Kerstin, Brioude Arnaud, Salles Vincent
Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Laboratoire des Multimatériaux et Interfaces, F-69622 Villeurbanne, France.
Faculté d'Odontologie, Université Claude Bernard Lyon 1, Lyon, France.
ACS Biomater Sci Eng. 2018 Dec 10;4(12):3927-3938. doi: 10.1021/acsbiomaterials.8b00810. Epub 2018 Oct 23.
In recent years, additive manufacturing (AM) technologies have attracted significant interest in many industrial and research fields, particularly in tissue engineering. Printed structures used as physical and bioactive supports for tissue regeneration are becoming increasingly complex so as to mimic natural tissues in order to answer future medical needs. Reproducing the biological environment of a native tissue from the microscopic to the macroscopic scale appears to be the best strategy for effective regeneration. Recent advances in AM have led to the production of scaffolds designed with a high precision. This Review presents results concerning two AM technologies which enable the highest accuracy of scaffold design to be obtained, with a precision down to the nanoscale. The first technique is based on a two-photon polymerization (TPP) process, while the other is based on a direct-writing electrospinning (DWES) system. Here, we present an overview of the fabrication mechanisms, the final scaffold properties, and their applications in tissue engineering. The production of highly resolved structures offers new possibilities for studying cell behavior in a controlled environment and also for adjusting the desired scaffold properties to address current and future needs in tissue engineering. The current technical limitations and future challenges are thus also discussed in this Review.
近年来，增材制造（AM）技术在许多工业和研究领域引起了广泛关注，尤其是在组织工程领域。用作组织再生的物理和生物活性支撑的打印结构正变得越来越复杂，以便模仿天然组织以满足未来的医疗需求。从微观到宏观尺度再现天然组织的生物环境似乎是有效再生的最佳策略。增材制造的最新进展已导致生产出高精度设计的支架。本综述介绍了有关两种增材制造技术的结果，这两种技术能够实现最高精度的支架设计，精度可达纳米级。第一种技术基于双光子聚合（TPP）工艺，而另一种基于直写静电纺丝（DWES）系统。在此，我们概述了制造机制、最终支架特性及其在组织工程中的应用。高分辨率结构的生产为在可控环境中研究细胞行为以及调整所需支架特性以满足组织工程当前和未来需求提供了新的可能性。因此，本综述还讨论了当前的技术局限性和未来挑战。
