Stevanović Milena, Đošić Marija, Janković Ana, Kojić Vesna, Vukašinović-Sekulić Maja, Stojanović Jovica, Odović Jadranka, Crevar Sakač Milkica, Rhee Kyong Yop, Mišković-Stanković Vesna
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Institute for Technology of Nuclear and Other Mineral Raw Materials (ITNMS), Bulevar Franš d'Eperea 86, 11000 Belgrade, Serbia.
ACS Biomater Sci Eng. 2018 Dec 10;4(12):3994-4007. doi: 10.1021/acsbiomaterials.8b00859. Epub 2018 Nov 2.
Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against and , pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
通过应用电泳沉积（EPD）技术，成功评估了抗生素庆大霉素（Gent）、天然聚合物壳聚糖（CS）和羟基磷灰石（HAP）的复合涂层。在优化的沉积条件（5V，12分钟）下，在纯钛板上进行EPD，以从三组分水悬浮液中一步获得具有良好抗菌性能的HAP/CS和HAP/CS/Gent复合涂层。通过X射线衍射（XRD）、场发射扫描电子显微镜、傅里叶变换红外光谱、热重分析和X射线光电子分析对复合涂层进行了表征，证实了在钛表面形成了复合HAP/CS和HAP/CS/Gent涂层，这是由于分子间氢键所致。采用XRD技术，通过获得特征衍射最大值检测到了HAP。证实了负载抗生素的复合涂层（HAP/CS/Gent）对[具体细菌1]和[具体细菌2]具有良好的抗菌活性，表明其在生物应用方面具有很高的潜力。在HAP/CS/Gent涂层中引入庆大霉素在测试的MRC-5和L929细胞系中引起了非常轻微的细胞毒性。使用MTT测试评估细胞活力，HAP/CS被归类为无细胞毒性。
