Sunairi M, Watabe K, Takagi M, Yano K
J Bacteriol. 1984 Dec;160(3):1037-40. doi: 10.1128/jb.160.3.1037-1040.1984.
In an n-alkane-assimilating Candida sp., transfer from glucose- to n-alkane-containing medium induced changes in the microsomal proteins, and several distinctive polypeptides were demonstrated in the solubilized microsomal fraction derived from n-alkane-grown cells. Long-term-labeling and pulse-labeling experiments in vivo demonstrated the synthesis of the specific microsomal polypeptides. The polypeptides were synthesized as in vitro translation products directed by polyadenylated RNA extracted from n-alkane-grown cells. Two major polypeptides were partially purified from the microsomal fraction from n-alkane-grown cells, and antiserum was prepared in a rabbit. Immunoprecipitation of these two polypeptides was accompanied by an increase in the amount of translatable mRNA. The molecular weights of the polypeptides derived from long-term-labeling, pulse-labeling and in vitro translation experiments appeared to be identical.
在一株同化正构烷烃的假丝酵母中，从含葡萄糖的培养基转移至含正构烷烃的培养基会引起微粒体蛋白的变化，并且在源自正构烷烃培养细胞的溶解微粒体部分中证实了几种独特的多肽。体内长期标记和脉冲标记实验证明了特定微粒体多肽的合成。这些多肽是由从正构烷烃培养细胞中提取的聚腺苷酸化RNA作为体外翻译产物合成的。从正构烷烃培养细胞的微粒体部分中部分纯化了两种主要多肽，并在兔中制备了抗血清。这两种多肽的免疫沉淀伴随着可翻译mRNA量的增加。长期标记、脉冲标记和体外翻译实验得到的多肽的分子量似乎是相同的。
