Department of Agronomy, IFAS, University of Florida, Gainesville, FL, USA.
Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, India.
Plant Cell Rep. 2021 Apr;40(4):595-604. doi: 10.1007/s00299-020-02656-3. Epub 2021 Jan 10.
Base editing is one of the promising genome editing tools for generating single-nucleotide changes in rice genome. Rice (Oryza sativa L.) is an important staple food crop, feeding half of the population around the globe. Developing new rice varieties with desirable agronomic traits is necessary for sustaining global food security. The use of genome editing technologies for developing rice varieties is pre-requisite in the present scenario. Among the genome editing technologies developed for rice crop improvement, base editing technology has emerged as an efficient and reliable tool for precise genome editing in rice plants. Base editing technology utilizes either adenosine or cytidine base editor for precise editing at the target region. A base editor (adenosine or cytidine) is a fusion of catalytically inactive CRISPR/Cas9 domain and adenosine or cytidine deaminase domain. In this review, authors have discussed the different adenine and cytosine base editors developed so far for precise genome editing of rice via base editing technology. We address the current progress, advances, limitations, as well as future perspectives of the base editing technology for rice crop improvement.
碱基编辑是一种很有前途的基因组编辑工具,可在水稻基因组中产生单核苷酸变化。水稻(Oryza sativa L.)是一种重要的主食作物,为全球一半的人口提供食物。培育具有理想农艺性状的新型水稻品种对于保障全球粮食安全至关重要。在当前情况下,利用基因组编辑技术开发水稻品种是必要的。在为提高水稻作物而开发的基因组编辑技术中,碱基编辑技术已成为在水稻植物中进行精确基因组编辑的有效且可靠的工具。碱基编辑技术利用腺嘌呤或胞嘧啶碱基编辑器在目标区域进行精确编辑。碱基编辑器(腺嘌呤或胞嘧啶)是无活性的 CRISPR/Cas9 结构域和腺嘌呤或胞嘧啶脱氨酶结构域的融合。在这篇综述中,作者讨论了迄今为止为通过碱基编辑技术对水稻进行精确基因组编辑而开发的不同腺嘌呤和胞嘧啶碱基编辑器。我们介绍了碱基编辑技术在提高水稻作物方面的当前进展、进展、局限性以及未来展望。