International Rice Research Institute, Manila, Philippines.
Dahlem Center of Plant Sciences Freie Universität, Berlin, Germany.
Methods Mol Biol. 2021;2238:135-143. doi: 10.1007/978-1-0716-1068-8_9.
The fast-moving CRISPR technology has allowed plant scientists to manipulate plant genomes in a targeted manner. So far, most of the applications were focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present a cytidine deaminase base editing method for editing the point mutations that control the grain size and seed coat color in rice.
快速发展的 CRISPR 技术使植物科学家能够以靶向方式对植物基因组进行操作。到目前为止，大多数应用都集中在通过创建插入缺失来敲除基因。然而，在育种计划中引入功能性单核苷酸多态性 (SNP) ，需要更精确的基因组编辑工具来辅助。CRISPR 碱基编辑工具就是为了满足这一需求而开发的。在本章中，我们介绍了一种胞嘧啶脱氨酶碱基编辑方法，用于编辑控制水稻粒大小和种皮颜色的点突变。
