Yadav Pranjali, Zhang Cheng, Whittaker Andrew K, Kailasam Kamalakannan, Shanavas Asifkhan
Inorganic & Organic Nanomedicine lab, Institute of Nano Science and Technology, Habitat Centre, Sector 64, Phase 10, Mohali, Punjab 160062, India.
Advanced Functional Nanomaterials lab, Institute of Nano Science and Technology, Habitat Centre, Sector 64, Phase 10, Mohali, Punjab 160062, India.
ACS Biomater Sci Eng. 2019 Dec 9;5(12):6590-6601. doi: 10.1021/acsbiomaterials.9b01224. Epub 2019 Nov 4.
A mesoporous magnetic nanohybrid functionalized with 14 wt % carbon nitride (CN) and loaded with curcumin (Cur) has been developed as a combination platform for photodynamic therapy and magnetic hyperthermia. CN-Cur complexes on the nanoparticle surface facilitate fast charge separation of hole-electron pairs under blue LED light irradiation and subsequent singlet oxygen generation. Cur release from the nanoparticle was significant only when exposed to both lysosomal pH (pH = 5.2) and an alternating current magnetic field (AMF). The mesoporous magnetic carbon nitride (MMCN) caused a 350% increase in the level of intracellular ROS as compared to the light exposed untreated control group. The nanohybrid was non-hemolytic and found to be biocompatible with HUVEC cells at concentrations up to 360 μg/mL. A similar concentration under AMF exposure caused a localized temperature rise of 4.2 °C and resulted in a 60% reduction in C6 cell viability. The cancer cell death further increased up to 80% under sequential exposure to light and AMF. The combinatorial treatment exerted significant cytoskeletal and nuclear damage in the cancer cells as assessed by confocal microscopy. The nanohybrid also exhibited relaxivity of 88 mM s, imparting significant weighted contrast to the cancer cells.
一种负载姜黄素（Cur）且功能化有14 wt%氮化碳（CN）的介孔磁性纳米杂化物已被开发为光动力疗法和磁热疗的联合平台。纳米颗粒表面的CN-Cur复合物在蓝色发光二极管光照下促进空穴-电子对的快速电荷分离并随后产生单线态氧。仅当暴露于溶酶体pH值（pH = 5.2）和交变磁场（AMF）时，姜黄素才会从纳米颗粒中显著释放。与未处理的光照对照组相比，介孔磁性氮化碳（MMCN）使细胞内活性氧水平增加了350%。该纳米杂化物无溶血作用，并且发现在浓度高达360 μg/mL时与脐静脉内皮细胞（HUVEC）具有生物相容性。在AMF暴露下，类似的浓度导致局部温度升高4.2°C，并使C6细胞活力降低60%。在依次暴露于光和AMF后，癌细胞死亡率进一步增加至80%。通过共聚焦显微镜评估，联合治疗对癌细胞造成了显著的细胞骨架和细胞核损伤。该纳米杂化物还表现出88 mM s的弛豫率，赋予癌细胞显著的加权对比度。
