Feltham Tyler, Paudel Sharada, Lobao Mario, Schon Lew, Zhang Zijun
Philadelphia College of Osteopathic Medicine-Georgia, Suwanee, Georgia, USA.
Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.
Ultrasound Med Biol. 2021 Apr;47(4):1045-1053. doi: 10.1016/j.ultrasmedbio.2020.12.019. Epub 2021 Jan 8.
This study was designed to investigate how low-intensity pulsed ultrasound (LIPUS) suppresses traumatic joint inflammation and thereafter affects the progression of posttraumatic osteoarthritis. Intra-articular fracture (IAF) was created in the right knee of rats. LIPUS was applied to the knees with IAFs for 20 min/d for 2 wk-LIPUS(+) group. The study controls included rats that underwent sham surgery but no LIPUS treatment (control group) or underwent IAF surgery without LIPUS treatment-LIPUS(-) group. By histology, at 4 wk, leukocyte infiltration in the synovium was reduced in the LIPUS(+) group. Furthermore, LIPUS treatment reduced CD68 macrophages in the synovium and limited their distribution mostly in the subintimal synovium. Measured with enzyme-linked immunosorbent assay, interleukin-1β (IL-1β) in the joint fluid of the LIPUS(+) group was reduced to about one-third that in the LIPUS(-) group. By reducing synovial macrophages and lowering IL-1β in the joint fluid, LIPUS is potentially therapeutic for posttraumatic osteoarthritis.
本研究旨在探讨低强度脉冲超声（LIPUS）如何抑制创伤性关节炎症，进而影响创伤后骨关节炎的进展。在大鼠右膝制造关节内骨折（IAF）。对发生IAF的膝关节应用LIPUS，每天20分钟，持续2周-LIPUS（+）组。研究对照包括接受假手术但未接受LIPUS治疗的大鼠（对照组）或接受IAF手术但未接受LIPUS治疗的大鼠-LIPUS（-）组。组织学检查显示，在4周时，LIPUS（+）组滑膜中的白细胞浸润减少。此外，LIPUS治疗减少了滑膜中CD68巨噬细胞，并将其分布主要限制在内膜下滑膜。通过酶联免疫吸附测定法测量，LIPUS（+）组关节液中的白细胞介素-1β（IL-1β）降至LIPUS（-）组的约三分之一。通过减少滑膜巨噬细胞和降低关节液中的IL-1β，LIPUS对创伤后骨关节炎具有潜在的治疗作用。
