局部注射间充质干细胞通过调节十二指肠穿孔中VEGF和CD31的表达来优化血管生成。
Locally injected Mesenchymal Stem Cells optimize angiogenesis by regulating VEGF and CD31 expression in duodenal perforation.
作者信息
Setiawan Eko, Purwanto Bambang, Wasita Brian, Putra Agung
机构信息
Doctorate Student of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
Department of Surgery, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia.
出版信息
Ann Med Surg (Lond). 2022 Sep 2;82:104529. doi: 10.1016/j.amsu.2022.104529. eCollection 2022 Oct.
BACKGROUND
Duodenal perforation is considered as one of gastrointestinal emergency with high morbidity and mortality rate. The MSCs have the ability to improve wound healing by releasing several growth factors and anti-inflammatory cytokines to promote the angiogenesis process. This study aimed to investigate the role of MSCs in duodenal perforation wound healing.
METHODS
MSCs were isolated from rat umbilical cord and injected into duodenal wound site at doses of 1.5x10 [(Putra et al., 2018) 66 cells for T1 group and 3x10 [(Putra et al., 2018) 66 cells for T2 group. The control group was treated by local injection of normal saline. The VEGF levels were measured by Western blot, while CD31 expression was analyzed using immunohistochemistry staining. All examinations were assessed on days 3 and 7.
RESULTS
Results showed a significant increase in VEGF and CD31 expression on days 3 and 7 (p < 0,05). The VEGF level was significantly decreased on day 7 compared to day 3.
CONCLUSION
The administration of MSCs improved the angiogenesis process in duodenal perforation by enhancing VEGF and CD31 expression.
背景
十二指肠穿孔被认为是一种发病率和死亡率较高的胃肠道急症。间充质干细胞（MSCs）能够通过释放多种生长因子和抗炎细胞因子来促进血管生成过程，从而改善伤口愈合。本研究旨在探讨间充质干细胞在十二指肠穿孔伤口愈合中的作用。
方法
从大鼠脐带中分离间充质干细胞，并以1.5×10[（Putra等人，2018）66个细胞]的剂量注入十二指肠伤口部位作为T1组，以3×10[（Putra等人，2018）66个细胞]的剂量注入作为T2组。对照组通过局部注射生理盐水进行治疗。采用蛋白质免疫印迹法测定血管内皮生长因子（VEGF）水平，采用免疫组织化学染色分析CD31表达。所有检查均在第3天和第7天进行评估。
结果
结果显示，在第3天和第7天，VEGF和CD31表达显著增加（p<0.05）。与第3天相比，第7天VEGF水平显著降低。
结论
间充质干细胞的应用通过增强VEGF和CD31表达改善了十二指肠穿孔的血管生成过程。
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