Al-Dhabaan Fahad A
Dept. of Biology, Science and Humanities College, Shaqra University, Alquwayiyah, Saudi Arabia.
Saudi J Biol Sci. 2021 Jan;28(1):73-77. doi: 10.1016/j.sjbs.2020.08.033. Epub 2020 Aug 29.
Crude oil biodegrading microorganism considers the key role for environmental preserving. In this investigation, crude oil biodegrading fungal strains have been isolated in polluted soil of crude-oil at khurais oil ground in Kingdom of Saudi Arabia. Among of 22 fungal isolates, only three isolates reflected potential capability for oil degradation. These isolates were identified and submitted to GenBank as (A1) (MT448790, (A2) (MT448791 and (A3) (MT459302) through internal-transcribed spacer-regions (ITS1&ITS2) for sequencing in molecular marker. Comparing with controls, strain (A1) was superior for biodegradation ability (58%) comparing with and degrading were showed 47 and 51% respectively. Employed CO evolution as indicator for petroleum oil biodegradation by the fungal isolates reflected that, emission highest CO2 (28.6%) comparing with and which showed 13% and 12.4% respectively. capability of sp. to tolerate and adapted oil pollutants with successful growth rate on them, indicated that it can be employed as mycoremediation agent for recovering restoring ecosystem when contaminated by crude oil.
原油降解微生物对环境保护起着关键作用。在本研究中，已从沙特阿拉伯王国胡赖斯油田受原油污染的土壤中分离出原油降解真菌菌株。在22株真菌分离物中，只有3株分离物表现出降解石油的潜在能力。通过内部转录间隔区（ITS1和ITS2）对这些分离物进行分子标记测序，并将其鉴定结果提交至GenBank，分别为（A1）（MT448790）、（A2）（MT448791）和（A3）（MT459302）。与对照相比，菌株（A1）的生物降解能力更强（58%），而（A2）和（A3）的降解率分别为47%和51%。以二氧化碳释放量作为真菌分离物对石油生物降解的指标，结果表明，（A1）释放的二氧化碳量最高（28.6%），而（A2）和（A3）分别为13%和12.4%。某菌株耐受和适应石油污染物并在其上成功生长的能力，表明其可作为一种真菌修复剂，用于在原油污染后恢复生态系统。
