Al-Dhabaan Fahad A
Department of Biology, Science and Humanities College, Shaqra University, Alquwayiyah, Saudi Arabia.
Saudi J Biol Sci. 2021 Oct;28(10):5786-5792. doi: 10.1016/j.sjbs.2021.06.030. Epub 2021 Jun 17.
Twenty five yeasts isolated were isolated from Khurais oil field in Saudi Arabia and assayed to evaluate their biodegradability. Only five isolates (namely, A1, A2, A3, A4 and A5) showed potential use of oil as sole carbon source. During incubation period, highest growth rate were recorded for A1, A2 and A3 isolates. Low growth distinguished A4 isolate; A5 isolate could not degrade oil. Spectrophotometrical analysis for four yeast isolates biodegradation activities indicated that, A1 isolate was superior for oil degradation (61%) comparing with A4 isolate which reflected lowest degradation % (33%). A2 and A3 isolates showed moderate biodegradation activity (56 and 51% respectively). D1/D2 domain of the 26S rRNA gene sequence was used as molecular marker to identify five yeast isolates. After comparing 26S rRNA gene sequences of five yeast isolates with highly similarity isolates, five yeast isolates (A1, A2, A3, A4 and A5)were submitted to database as (MW488263), (MW488264), (MW488265) and (MW488266) respectively. Using OXF1/ACR1 primer, specific lipase gene amplicon with 250 bp were detected with in all four yeast isolates.
从沙特阿拉伯的胡赖斯油田分离出25株酵母，并对其进行分析以评估它们的生物降解能力。只有五株分离株（即A1、A2、A3、A4和A5）显示出有将油作为唯一碳源的潜力。在培养期间，A1、A2和A3分离株的生长速率最高。A4分离株生长缓慢；A5分离株不能降解油。对四株酵母分离株的生物降解活性进行分光光度分析表明，与降解率最低（33%）的A4分离株相比，A1分离株的油降解能力更强（61%）。A2和A3分离株表现出中等的生物降解活性（分别为56%和51%）。26S rRNA基因序列的D1/D2结构域被用作分子标记来鉴定五株酵母分离株。将五株酵母分离株的26S rRNA基因序列与高度相似的分离株进行比较后，五株酵母分离株（A1、A2、A3、A4和A5）分别以（MW488263）、（MW488264）、（MW488265）和（MW488266）的名称提交到数据库。使用OXF1/ACR1引物，在所有四株酵母分离株中均检测到了250 bp的特异性脂肪酶基因扩增子。
