Inoue T, Muraoka Y, Fukushima K, Shimozawa R
Department of Chemistry, Faculty of Science, Fukuoka University, Japan.
Chem Phys Lipids. 1988 Feb;46(2):107-15. doi: 10.1016/0009-3084(88)90120-x.
The effect of surfactants on the "fluidity" of dipalmitoylphosphatidylcholine (DPPC) vesicle membrane was studied by means of the fluorescence depolarization technique with fatty acid fluorescent probes, in which the anthroyloxy group is introduced at different positions along the acyl chain. Three types of surfactants were examined; anionic sodium alkylsulfates, cationic alkyltrimethylammonium chlorides, and non-ionic alkanoyl-N-methylglucamides (MEGA-n). Perturbing effects of the surfactants depended on both the alkyl chain-length and the type of head group. Sodium alkylsulfates with octyl- and decyl-chain and alkyltrimethylammonium chlorides with octyl-, decyl- and dodecyl-chain did not affect the membrane fluidity when incorporated in the membrane, whereas sodium dodecylsulfate and tetradecyltrimethylammonium chloride decreased the membrane fluidity at both gel and liquid crystalline states of the membrane. All the MEGA series surfactants decreased the membrane fluidity, whose perturbing potency was in the order of MEGA-8 less than MEGA-9 approximately equal to MEGA-10. The perturbation at different depths in the membrane by sodium dodecylsulfate and MEGA-9 was also examined. No significant change in the fluidity gradient across the membrane was induced by the addition of these surfactants.
通过使用带有脂肪酸荧光探针的荧光去极化技术，研究了表面活性剂对二棕榈酰磷脂酰胆碱（DPPC）囊泡膜“流动性”的影响，其中蒽氧基沿着酰基链在不同位置引入。研究了三种类型的表面活性剂：阴离子型烷基硫酸钠、阳离子型烷基三甲基氯化铵和非离子型烷酰基 - N - 甲基葡糖酰胺（MEGA - n）。表面活性剂的扰动作用取决于烷基链长度和头基类型。具有辛基和癸基链的烷基硫酸钠以及具有辛基、癸基和十二烷基链的烷基三甲基氯化铵掺入膜中时不影响膜流动性，而十二烷基硫酸钠和十四烷基三甲基氯化铵在膜的凝胶态和液晶态均降低膜流动性。所有MEGA系列表面活性剂均降低膜流动性，其扰动效力顺序为MEGA - 8小于MEGA - 9约等于MEGA - 10。还研究了十二烷基硫酸钠和MEGA - 9在膜不同深度处的扰动情况。添加这些表面活性剂未引起膜上流动性梯度的显著变化。
