Tilley L, Thulborn K R, Sawyer W H
J Biol Chem. 1979 Apr 25;254(8):2592-4.
The rotational behavior of a set of n-(9-anthroyloxy) fatty acid fluorescent probes is examined in two liquid paraffins and in liposomes composed of dipalmitoyl phosphatidylcholine. As has been observed with other membrane fluorescent probes (Hare, F., and Lussan, C. (1977) Biochim. Biophys. Acta 467, 262-272), the degree of fluorescence depolarization for a given solvent viscosity is dependent on the solvent standard employed. In addition, when the anthroyloxy group is in the terminal position of the acyl chain, it has more rotational freedom than when it is conjugated to positions 6, 9, or 12 where the rotational motion of the fluorophore is similar. When incorporated into lipid bilayers, values of fluorescence polarization reflect the gradient of "fluidity" which extends from the surface to the center of the membrane. The nature of this polarization gradient is discussed in relation to the intrinsic differences between the probes and the anisotropic rotations responsible for depolarization.
研究了一组n-（9-蒽氧基）脂肪酸荧光探针在两种液体石蜡以及由二棕榈酰磷脂酰胆碱组成的脂质体中的旋转行为。正如其他膜荧光探针所观察到的那样（Hare, F., and Lussan, C. (1977) Biochim. Biophys. Acta 467, 262 - 272），对于给定的溶剂粘度，荧光去极化程度取决于所采用的溶剂标准。此外，当蒽氧基处于酰基链的末端位置时，它比与荧光团旋转运动相似的6、9或12位共轭时具有更多的旋转自由度。当掺入脂质双层中时，荧光偏振值反映了从膜表面到中心延伸的“流动性”梯度。结合探针之间的内在差异以及导致去极化的各向异性旋转，讨论了这种偏振梯度的性质。
