Samarina Lidiia S, Bobrovskikh Alexandr V, Doroshkov Alexey V, Malyukova Lyudmila S, Matskiv Alexandra O, Rakhmangulov Ruslan S, Koninskaya Natalia G, Malyarovskaya Valentina I, Tong Wei, Xia Enhua, Manakhova Karina A, Ryndin Alexey V, Orlov Yuriy L
Biotechnology Department, Federal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences, Sochi, Russia.
Institute Cytology and Genetics Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Front Genet. 2020 Dec 23;11:611283. doi: 10.3389/fgene.2020.611283. eCollection 2020.
Cold and drought are two of the most severe threats affecting the growth and productivity of the tea plant, limiting its global spread. Both stresses cause osmotic changes in the cells of the tea plant by decreasing their water potential. To develop cultivars that are tolerant to both stresses, it is essential to understand the genetic responses of tea plant to these two stresses, particularly in terms of the genes involved. In this study, we combined literature data with interspecific transcriptomic analyses (using and ) to choose genes related to cold tolerance. We identified 45 stress-inducible candidate genes associated with cold and drought responses in tea plants based on a comprehensive homologous detection method. Of these, nine were newly characterized by us, and 36 had previously been reported. The gene network analysis revealed upregulated expression in -related cluster of factors, connected genes (hexokinases, galactinol synthases, complex, etc.) indicating their possible co-expression. Using qRT-PCR we revealed that 10 genes were significantly upregulated in response to both cold and drought in tea plant: , and . were specifically upregulated in cold, while were specifically upregulated in drought. Interestingly, the expression of was higher in the recovery stage of both stresses, indicating its potentially important role in plant recovery after stress. In addition, some genes, such as , and , were significantly positively correlated between the cold and drought responses. , and , by contrast, showed significantly negative correlations between the cold and drought responses. Our results provide valuable information and robust candidate genes for future functional analyses intended to improve the stress tolerance of the tea plant and other species.
寒冷和干旱是影响茶树生长和产量的两个最严重威胁，限制了其在全球的传播。这两种胁迫都会通过降低茶树细胞的水势而导致渗透变化。为了培育对这两种胁迫都耐受的品种，了解茶树对这两种胁迫的遗传反应至关重要，特别是涉及的基因方面。在本研究中，我们将文献数据与种间转录组分析（使用 和 ）相结合，以选择与耐寒性相关的基因。我们基于全面的同源性检测方法，鉴定出45个与茶树冷害和干旱反应相关的胁迫诱导候选基因。其中，9个是我们新鉴定的，36个先前已有报道。基因网络分析显示，在 相关的 因子簇中表达上调， 连接的基因（己糖激酶、棉子糖合酶、 复合体等）表明它们可能共表达。使用qRT-PCR我们发现，10个基因在茶树中对寒冷和干旱都有显著上调表达： 、 和 。 在寒冷条件下特异性上调，而 在干旱条件下特异性上调。有趣的是， 在两种胁迫的恢复阶段表达更高，表明其在胁迫后植物恢复中可能具有重要作用。此外，一些基因，如 、 和 ，在冷害和干旱反应之间显著正相关。相比之下， 、 和 在冷害和干旱反应之间显示出显著负相关。我们的结果为未来旨在提高茶树和其他物种胁迫耐受性的功能分析提供了有价值的信息和可靠的候选基因。
