一种用于研究癌症相关成纤维细胞在肿瘤生长中作用的小鼠模型。
A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth.
机构信息
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles.
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles; Department of Molecular Biology, Princeton University.
出版信息
J Vis Exp. 2020 Dec 22(166). doi: 10.3791/61883.
Cancer-associated fibroblasts (CAFs) can play an important role in tumor growth by creating a tumor-promoting microenvironment. Models to study the role of CAFs in the tumor microenvironment can be helpful for understanding the functional importance of fibroblasts, fibroblasts from different tissues, and specific genetic factors in fibroblasts. Mouse models are essential for understanding the contributors to tumor growth and progression in an in vivo context. Here, a protocol in which cancer cells are mixed with fibroblasts and introduced into mice to develop tumors is provided. Tumor sizes over time and final tumor weights are determined and compared among groups. The protocol described can provide more insight into the functional role of CAFs in tumor growth and progression.
癌症相关成纤维细胞（CAFs）可以通过创建肿瘤促进微环境在肿瘤生长中发挥重要作用。研究 CAFs 在肿瘤微环境中的作用的模型有助于理解成纤维细胞、不同组织来源的成纤维细胞以及成纤维细胞中特定遗传因素的功能重要性。小鼠模型对于理解体内肿瘤生长和进展的贡献因素至关重要。这里提供了一种将癌细胞与成纤维细胞混合并引入小鼠中以形成肿瘤的方案。随着时间的推移，测定并比较各组肿瘤的大小和最终肿瘤重量。所描述的方案可以更深入地了解 CAFs 在肿瘤生长和进展中的功能作用。
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