Gil Sara, Silva Joana M, Mano João F
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence of Tissue Engineering and Regenerative Medicine, Avepark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal.
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
ACS Biomater Sci Eng. 2015 Oct 12;1(10):1016-1025. doi: 10.1021/acsbiomaterials.5b00292. Epub 2015 Sep 1.
Self-standing nanocomposite films based on biopolymers and functional nanostructures have been widely used due to their potential applications as active elements in biomedical devices. The coupling between chitosan (CHI) and alginate (ALG) multilayered films and magnetic nanoparticles (MNPs) allowed to fabricate magnetic responsive freestanding membranes with a high structural control along the thickness, using the layer-by-layer (LbL) methodology. The mechanical characterization evidenced a trend for an increase of both Young modulus, and ultimate tensile strength with the inclusion of MNPs, or by cross-linking with genipin. Additionally, the multilayered membranes exhibited shape memory properties triggered by hydration. The in vitro biological performance studies showed that cells were more viable and adherent with higher proliferation rates when MNPs were included in the membranes. Our results suggested the potential of the developed magneto-active freestanding membranes for biomedical applications, such as in tissue engineering and biomedical applications.
基于生物聚合物和功能性纳米结构的自支撑纳米复合薄膜，因其作为生物医学设备中的活性元件的潜在应用而被广泛使用。壳聚糖（CHI）和藻酸盐（ALG）多层膜与磁性纳米颗粒（MNP）之间的耦合，使得能够使用逐层（LbL）方法制备沿厚度方向具有高度结构可控性的磁响应独立膜。力学表征表明，随着MNP的加入或与京尼平交联，杨氏模量和极限拉伸强度均有增加的趋势。此外，多层膜表现出由水合作用触发的形状记忆特性。体外生物学性能研究表明，当膜中包含MNP时，细胞更具活力且粘附性更好，增殖速率更高。我们的结果表明，所开发的磁活性独立膜在组织工程和生物医学应用等生物医学领域具有潜在应用价值。
