Liang Hongshan, Li Jing, He Yun, Xu Wei, Liu Shilin, Li Yan, Chen Yijie, Li Bin
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan 430070, China.
ACS Biomater Sci Eng. 2016 Mar 14;2(3):317-325. doi: 10.1021/acsbiomaterials.5b00363. Epub 2016 Feb 2.
Here, we reported a designable pH-responsive system on the basis of coordination bonded metal-tannic acid (TA) networks on zein/quaternized chitosan (HTCC) nanoparticles (NPs). Zein/HTCC NPs has been developed as a promising delivery system for natural bioactives in our lab previously. The coordination bonds between either the "NH-metal" or the "metal-TA" are sensitive to pH variations that would release encapsulated drugs under designated pH conditions. Metal-TA film-coated zein/HTCC NPs had a spherical structure with particle size of 110-120 nm. Doxorubicin (DOX) was used as a model anticancer drug for release study and cell viability. The in vitro release profile of DOX loaded metal-TA films coated zein/HTCC NPs (DOX-zein/HTCC-TA/metal NPs) showed a significant pH-responsiveness when changing the amount or the type of metal ions. In in vitro cell assays, the blank zein/HTCC-TA/metal NPs showed low cytotoxicity, but the DOX-loaded NPs exhibited a high cytotoxic activity against HepG2. To impart enhanced imaging properties of metal-TA films, we used Eu to chelate with ligand named 2-thenoyltrifluoroacetone (TTA) to intense fluorescence intensities of Eu-TA films so as to develop pH-responsive zein/HTCC-TA/metal NPs for anticancer drug delivery as well as cell imaging.
在此，我们报道了一种基于玉米醇溶蛋白/季铵化壳聚糖（HTCC）纳米颗粒（NPs）上配位键合的金属-单宁酸（TA）网络的可设计pH响应系统。玉米醇溶蛋白/HTCC NPs此前已在我们实验室开发成为一种用于天然生物活性物质的有前景的递送系统。“NH-金属”或“金属-TA”之间的配位键对pH变化敏感，这会在指定pH条件下释放封装的药物。金属-TA膜包被的玉米醇溶蛋白/HTCC NPs具有球形结构，粒径为110-120 nm。阿霉素（DOX）用作释放研究和细胞活力的模型抗癌药物。负载DOX的金属-TA膜包被的玉米醇溶蛋白/HTCC NPs（DOX-玉米醇溶蛋白/HTCC-TA/金属NPs）的体外释放曲线在改变金属离子的量或类型时显示出显著的pH响应性。在体外细胞试验中，空白玉米醇溶蛋白/HTCC-TA/金属NPs显示出低细胞毒性，但负载DOX的NPs对HepG2表现出高细胞毒性活性。为了赋予金属-TA膜增强的成像特性，我们使用铕与名为2-噻吩甲酰三氟丙酮（TTA）的配体螯合，以增强铕-TA膜的荧光强度，从而开发用于抗癌药物递送以及细胞成像的pH响应性玉米醇溶蛋白/HTCC-TA/金属NPs。
