Kulkarni Ketav, Hung Juichien, Fulcher Alex J, Chan Alex H P, Hong Andrew, Forsythe John S, Aguilar Marie-Isabel, Wise Steven G, Del Borgo Mark P
Heart Research Institute, Newtown, New South Wales 2042, Australia.
Sydney Medical School, Sydney University, Sydney, New South Wales 2006, Australia.
ACS Biomater Sci Eng. 2018 Nov 12;4(11):3843-3847. doi: 10.1021/acsbiomaterials.8b01065. Epub 2018 Oct 3.
β-peptides uniquely form shear thinning hydrogels which are proteolytically stable and biocompatible. Herein we describe the synthesis, material and optical characterization of a new class of fluorescently labeled hydrogelators based on a helical -acetylated β-peptide backbone. The resulting hydrogels were analyzed using fluorescence microscopy to confirm successful incorporation of the fluorophore within the fiber matrix without compromising the β-peptide self-assembly. Serial, noninvasive conscious animal imaging was used to monitor the injected hydrogel, delivered via subcutaneous injection, while tracking their degradation patterns in real-time. The hydrogels demonstrated persistent, high-intensity fluorescence when monitored over a 14-day period.
β-肽独特地形成剪切变稀水凝胶，这种水凝胶具有蛋白水解稳定性和生物相容性。在此，我们描述了基于螺旋状乙酰化β-肽主链的一类新型荧光标记水凝胶剂的合成、材料和光学表征。使用荧光显微镜对所得水凝胶进行分析，以确认荧光团成功掺入纤维基质中，同时不影响β-肽的自组装。通过连续的、非侵入性的清醒动物成像来监测经皮下注射递送的注入水凝胶，同时实时追踪它们的降解模式。在14天的监测期内，这些水凝胶表现出持续的高强度荧光。
