Cox Sophie C, Jamshidi Parastoo, Eisenstein Neil M, Webber Mark A, Burton Hanna, Moakes Richard J A, Addison Owen, Attallah Moataz, Shepherd Duncan E T, Grover Liam M
Royal Centre for Defence Medicine, Birmingham Research Park, Vincent Drive, Edgbaston B15 2SQ, United Kingdom.
Institute of Food Research, Norwich Research Park, Norwich NR4 7UG, United Kingdom.
ACS Biomater Sci Eng. 2017 Aug 14;3(8):1616-1626. doi: 10.1021/acsbiomaterials.7b00336. Epub 2017 Jun 28.
Additive manufacturing (AM) technologies enable greater geometrical design freedom compared with subtractive processes. This flexibility has been used to manufacture patient-matched implants. Although the advantages of AM are clear, the optimization at each process stage is often understated. Here we demonstrate that surface finishing of selective laser melted (SLM) implants significantly alters topography, which has implications for cellular and biofilm adhesion. Hot isostatic pressing of as-fabricated Ti-6Al-4V implants was shown to reduce porosity (1.04 to 0.02%) and surface roughness (34 ± 8 to 22 ± 3 μm). Despite these surface changes, preosteoblasts exhibited a similar viability and proliferation after 7 days of culture. Contrastingly, sandblasting and polishing significantly reduced cellular activity and increased cytotoxicity. Bacterial specimens (S and ) adhered more homogeneously to sandblasted implants compared with other treatments. This suggests that sandblasting may place the implant at risk of infection and reduce the strength of interaction with the surrounding soft tissues. The ability to tune the adhesion of cells to additively manufactured Ti-6Al-4V implants using postprocessing methods was demonstrated. Because the degree of tissue integration required of implants is application specific, these methods may be useful to tailor osseointegration. However, surface competition between mammalian and bacterial cells remains a challenge.
与减法加工工艺相比，增材制造（AM）技术能够实现更大的几何设计自由度。这种灵活性已被用于制造与患者匹配的植入物。尽管增材制造的优势显而易见，但每个工艺阶段的优化往往被低估。在此，我们证明选择性激光熔化（SLM）植入物的表面处理会显著改变其形貌，这对细胞和生物膜的粘附具有重要影响。结果表明，对制成的Ti-6Al-4V植入物进行热等静压可降低孔隙率（从1.04%降至0.02%）和表面粗糙度（从34±8μm降至22±3μm）。尽管表面发生了这些变化，但前成骨细胞在培养7天后仍表现出相似的活力和增殖能力。相比之下，喷砂和抛光显著降低了细胞活性并增加了细胞毒性。与其他处理方式相比，细菌标本（S和 ）在喷砂处理的植入物上的粘附更为均匀。这表明喷砂处理可能会使植入物面临感染风险，并降低与周围软组织的相互作用强度。研究证明了使用后处理方法调节细胞对增材制造的Ti-6Al-4V植入物粘附的能力。由于植入物所需的组织整合程度因应用而异，这些方法可能有助于定制骨整合。然而，哺乳动物细胞和细菌细胞之间的表面竞争仍然是一个挑战。
