Nikravesh Niusha, Cox Sophie C, Ellis Marianne J, Grover Liam M
School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
School of Chemical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom.
ACS Biomater Sci Eng. 2017 Aug 14;3(8):1750-1757. doi: 10.1021/acsbiomaterials.7b00191. Epub 2017 Jul 3.
This study presents experimental data of a fluidized-bed bioreactor for the cultivation of encapsulated pancreatic beta-cells. The fluidization quality for the bioreactor was evaluated at different flow rate using bed-expansion parameters. Homogeneous distribution of microcapsules was achieved at a flow rate of 2000 μL/min. This enabled efficient contact between the encapsulated cells and medium, which contributed to high cell viability. Microcapsule breakage was <4% on day 7 and confirmed the stability of encapsulated systems under fluidized culture. Importantly, endocrine beta-cells cultured in the bioreactor were shown to be dramatically more responsive to changes in glucose concentration compared to static culture ( < 0.001). On the basis of these results, cultivation of encapsulated cells in a fluidized bioreactor, especially for pancreatic beta-cells that are limited in supply, is a promising approach to address the lack of a safe method for storage and handling of cells between laboratories and clinical sites prior to transplantation.
本研究展示了用于培养包封化胰岛β细胞的流化床生物反应器的实验数据。使用床层膨胀参数在不同流速下评估了生物反应器的流化质量。在流速为2000 μL/分钟时实现了微胶囊的均匀分布。这使得包封化细胞与培养基之间能够有效接触，从而有助于保持较高的细胞活力。在第7天微胶囊破损率<4%，证实了包封系统在流化培养条件下的稳定性。重要的是，与静态培养相比，在生物反应器中培养的内分泌β细胞对葡萄糖浓度变化的反应明显更强（<0.001）。基于这些结果，在流化床生物反应器中培养包封化细胞，特别是对于供应有限的胰岛β细胞，是一种有前景的方法，可解决在移植前实验室和临床场所之间缺乏安全的细胞储存和处理方法的问题。
