Zhou Jianhong, Li Bo, Zhao Lingzhou, Zhang Lan, Han Yong
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Institute of Physics & Optoelectronics Technology, Baoji University of Arts and Sciences, Baoji 721016, China.
ACS Biomater Sci Eng. 2017 Jul 10;3(7):1437-1450. doi: 10.1021/acsbiomaterials.6b00710. Epub 2017 Jan 9.
Advanced titanium (Ti) based bone implants with antibacterial and osteogenic activities are extremely needed in clinics. Herein, biomimetic hierarchical micropore/nanorod patterned coatings (MNRs) on Ti were developed, in which the nanorods revealed a fixed interrod spacing of about 70 nm and consisted of fluorine (F) incorporated CaSr(PO)(OH) (Sr-HA, strontium containing hydroxyapatite) with the fixed Sr but different F content. The incorporation of F was determined by XRD, FTTR, and TEM, revealing the substitution of OH in Sr-HA to F. The topography, surface roughness, and hydrophilic nature of MNRs were not apparently affected by the incorporation of F. The antibacterial and osteogenic activities of MNRs were assessed and in a bacterial-infected rabbit model. Both the and results showed that F-free and ∼1 wt % F doped MNR significantly promoted osteogenic activity compared to that of Ti but showed a lack of antibacterial activity. The incorporation of a higher dose of F led to the significantly improved antibacterial and osteogenic activities, and the effects were related to the incorporated F dose. In total, compared to F-free, ∼1, ∼2, and ∼7 wt % F doped MNRs, ∼5 wt % F doped MNR significantly enhanced antibacterial and osteogenic activities, especially new bone formation and osseointegration in the bacteria-infected case, showing a high potential as the next generation of advanced bone implant.
临床上极其需要具有抗菌和成骨活性的先进钛（Ti）基骨植入物。在此，开发了Ti表面的仿生分级微孔/纳米棒图案涂层（MNRs），其中纳米棒的棒间间距固定约为70nm，由掺入氟（F）的CaSr(PO)(OH)（Sr-HA，含锶羟基磷灰石）组成，Sr含量固定但F含量不同。通过XRD、FTTR和TEM确定了F的掺入情况，揭示了Sr-HA中OH被F取代。F的掺入对MNRs的形貌、表面粗糙度和亲水性没有明显影响。在细菌感染的兔模型中评估了MNRs的抗菌和成骨活性。体外和体内结果均表明，与Ti相比，无氟和1wt%F掺杂的MNR显著促进了成骨活性，但缺乏抗菌活性。掺入更高剂量的F导致抗菌和成骨活性显著提高，且效果与掺入的F剂量有关。总体而言，与无氟、1、2和7wt%F掺杂的MNRs相比，~5wt%F掺杂的MNR显著增强了抗菌和成骨活性，尤其是在细菌感染情况下的新骨形成和骨整合，显示出作为下一代先进骨植入物的巨大潜力。
