纳米棒直径通过 Wnt/β-连环蛋白通路调节分级微孔/纳米棒图案化涂层的成骨活性。
Nanorod diameter modulated osteogenic activity of hierarchical micropore/nanorod-patterned coatings via a Wnt/β-catenin pathway.
机构信息
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, China; Institute of Physics & Optoelectronics Technology, Baoji University of Arts and Sciences, Baoji, China.
State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
出版信息
Nanomedicine. 2018 Jul;14(5):1719-1731. doi: 10.1016/j.nano.2018.04.006. Epub 2018 Apr 14.
Hierarchical micropore/nanorod-patterned strontium doped hydroxyapatite (CaSr(PO)(OH), Sr-HA) structures (MNRs) with different nanorod diameters of about 30, 70 and 150 nm were coated on titanium, to investigate the effect of nanorod diameter on osteogenesis and the involved mechanism. Compared to micropore/nanogranule-patterned Sr-HA coating (MNG), MNRs gave rise to dramatically enhanced in vitro mesenchymal stem cell functions including osteogenic differentiation in the absence of osteogenic supplements and in vivo osseointegration related to the nanorod diameter with about 70 nm displaying the best effects. MNRs activated the cellular Wnt/β-catenin pathway by increasing the expression of Wnt3a and LRP6 and decreasing the expression of Wnt/β-catenin pathway antagonists (sFRP1, sFRP2, Dkk1 and Dkk2). The exogenous Wnt3a significantly enhanced the β-catenin signaling activation and cell differentiation on MNG, and the exogenous Dkk1 attenuated the enhancing effect of MNRs on them. The data demonstrate that MNRs favor osseointegration via a Wnt/β-catenin pathway.
具有不同纳米棒直径（约 30、70 和 150nm）的分级微孔/纳米棒图案化锶掺杂羟基磷灰石（CaSr(PO)(OH)，Sr-HA）结构（MNRs）被涂覆在钛上，以研究纳米棒直径对成骨的影响及其相关机制。与微孔/纳米颗粒图案化 Sr-HA 涂层（MNG）相比，MNRs 显著增强了体外间充质干细胞功能，包括在没有成骨补充剂的情况下的成骨分化，以及与纳米棒直径相关的体内骨整合，其中约 70nm 的纳米棒显示出最佳效果。MNRs 通过增加 Wnt3a 和 LRP6 的表达并降低 Wnt/β-catenin 通路拮抗剂（sFRP1、sFRP2、Dkk1 和 Dkk2）的表达来激活细胞 Wnt/β-catenin 通路。外源性 Wnt3a 显著增强了 MNG 上的β-catenin 信号激活和细胞分化，而外源性 Dkk1 减弱了 MNRs 对其的增强作用。数据表明，MNRs 通过 Wnt/β-catenin 通路促进骨整合。
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