MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Department of Physics, Research Institute for Biomimetics and Soft Matter, Xiamen University, Xiamen 361005, China.
Chem Soc Rev. 2017 Jul 3;46(13):3962-3979. doi: 10.1039/c7cs00169j.
Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters. In particular, the shell-isolated nanostructure-enhanced fluorescence, an innovative new mode for plasmon-enhanced surface analysis, is included. These new developments are based on the coupling of the fluorophores in their excited states with localized surface plasmons in nanoparticles, where local field enhancement leads to improved brightness of molecular emission and higher detection sensitivity. Here, we review the recent progress in PEF with an emphasis on the mechanism of plasmon enhancement, substrate preparation, and some advanced applications, including an outlook on PEF with high time- and spatially resolved properties.
强荧光体的荧光光谱学是一种具有超高灵敏度的检测和成像工具，可达到单分子水平。等离子体增强荧光（PEF）不仅提供了增强的发射和减少的寿命，而且通过结合弱量子发射器，扩展了荧光的领域，避免了光漂白，并提供了分辨率明显优于衍射极限的成像机会。它还通过将金属纳米结构和量子发射器结合在一起，为一类新的光稳定探针打开了大门。特别是，包括了壳隔离纳米结构增强荧光，这是一种用于等离子体增强表面分析的创新新模式。这些新的发展基于在纳米粒子中的局部表面等离激元与荧光团在激发态中的耦合，其中局部场增强导致分子发射的亮度提高和检测灵敏度提高。在这里，我们重点介绍了等离子体增强的机制、衬底制备以及一些高级应用，综述了等离子体增强荧光的最新进展，包括对具有高时间和空间分辨率特性的等离子体增强荧光的展望。
