Articular cartilage experiences mechanical constraints leading to chondral defects that inevitably evolve into osteoarthritis (OA), because cartilage has poor intrinsic repair capacity. Although OA is an incurable degenerative disease, several dietary supplements may help improve OA outcomes. In this study, we investigated the effects of Dielen hydrolyzed fish collagens from skin (Promerim30 and Promerim60) and cartilage (Promerim40) to analyze the phenotype and metabolism of equine articular chondrocytes (eACs) cultured as organoids. Here, our findings demonstrated the absence of cytotoxicity and the beneficial effect of Promerim hydrolysates on eAC metabolic activity under physioxia; further, Promerim30 also delayed eAC senescence. To assess the effect of Promerim in a cartilage-like tissue, eACs were cultured as organoids under hypoxia with or without BMP-2 and/or IL-1β. In some instances, alone or in the presence of IL-1β, Promerim30 and Promerim40 increased protein synthesis of collagen types I and II, while decreasing transcript levels of proteases involved in OA pathogenesis, namely , and the metalloproteinases , and . Both Promerim hydrolysates also decreased Htra1 protein amounts, particularly in inflammatory conditions. The effect of Promerim was enhanced under inflammatory conditions, possibly due to a decrease in the synthesis of inflammation-associated molecules. Finally, Promerim favored in vitro repair in a scratch wound assay through an increase in cell proliferation or migration. Altogether, these data show that Promerim30 and 40 hold promise as dietary supplements to relieve OA symptoms in patients and to delay OA progression.