Computational and Chemical Biology, Fondazione Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.
Research Group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum-University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Molecules. 2021 Jan 7;26(2):262. doi: 10.3390/molecules26020262.
In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.
在目前这篇概念验证论文中，我们探索了一种常见的血液微采样固体支持物（干血斑，DBS）和一种设备（体积吸收微采样，VAMS）的潜力，该设备用于通过高分辨率 LC-MS/MS 对人全血进行非靶向脂质组学分析。通过 DBS 和 VAMS 获得的干血微样本用不同的溶剂组合提取，并与血液进行比较，以评估它们在最广泛地分析脂质化学空间方面的效率。尽管需要进一步努力来更好地描述这种方法，但我们的结果表明，VAMS 是一种可行的非靶向研究选择，其使用将带来脂质组学领域的所有相关已知优势，例如不受红细胞压积影响。
