Couacault Pauline, Avella Dennisse, Londoño-Osorio Sara, Lorenzo Ana S, Gradillas Ana, Kärkkäinen Olli, Want Elizabeth, Witting Michael
Metabolomics and Proteomics Core Helmholtz Zentrum München Neuherberg Germany.
Afekta Technologies Ltd. Kuopio Finland.
Anal Sci Adv. 2024 Jun 14;5(5-6):e2400002. doi: 10.1002/ansa.202400002. eCollection 2024 Jun.
Blood microsampling (BµS) offers an alternative to conventional methods that use plasma or serum for profiling human health, being minimally invasive and cost effective, especially beneficial for vulnerable populations. We present a non-systematic review that offers a synopsis of the analytical methods, applications and perspectives related to dry blood microsampling in targeted and untargeted metabolomics and lipidomics research in the years 2022 and 2023. BµS shows potential in neonatal and paediatric studies, therapeutic drug monitoring, metabolite screening, biomarker research, sports supervision, clinical disorders studies and forensic toxicology. Notably, dried blood spots and volumetric absorptive microsampling options have been more extensively studied than other volumetric technologies. Therefore, we suggest that a further investigation and application of the volumetric technologies will contribute to the use of BµS as an alternative to conventional methods. Conversely, we support the idea that harmonisation of the analytical methods when using BµS would have a positive impact on its implementation.
血液微量采样（BµS）为使用血浆或血清来分析人类健康状况的传统方法提供了一种替代方案，它具有微创性且成本效益高，对弱势群体尤其有益。我们进行了一项非系统性综述，概述了2022年和2023年在靶向和非靶向代谢组学及脂质组学研究中与干血微量采样相关的分析方法、应用及前景。BµS在新生儿和儿科研究、治疗药物监测、代谢物筛查、生物标志物研究、运动监督、临床疾病研究和法医毒理学方面显示出潜力。值得注意的是，干血斑和体积吸收性微量采样方法比其他体积技术得到了更广泛的研究。因此，我们建议进一步研究和应用体积技术将有助于BµS替代传统方法。相反，我们支持这样的观点，即使用BµS时分析方法的统一将对其应用产生积极影响。
