Rodríguez-Pires Silvia, Espeso Eduardo A, Rasiukevičiūtė Neringa, Melgarejo Paloma, De Cal Antonieta
Department of Plant Protection, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de La Coruña Km. 7, 28040 Madrid, Spain.
Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB), Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
J Fungi (Basel). 2021 Jan 7;7(1):32. doi: 10.3390/jof7010032.
Light represents a ubiquitous source of information for organisms to evaluate their environment. The influence of light on colony growth and conidiation was determined for three isolates. The highest mycelial growth rate was observed under red light for the three isolates, followed by green light, daylight or darkness. However, reduced sporulation levels were observed in darkness and red light, but conidiation enhancement was found under daylight, black and green light with more hours of exposure to light. Putative photoreceptors for blue (white-collar and cryptochromes), green (opsins), and red light (phytochromes) were identified, and the photoresponse-related regulatory family of velvet proteins. A unique ortholog for each photoreceptor was found, and their respective domain architecture was highly conserved. Transcriptional analyses of uncovered sets of genes were performed under daylight or specific color light, and both in time course illumination, finding light-dependent triggered gene expression of , , , and , and color light as a positive inductor of , , , and expression. has a highly conserved set of photoreceptors with other light-responsive fungi. Our phenotypic analyses and the existence of this light-sensing machinery suggest transcriptional regulatory systems dedicated to modulating the development and dispersion of this pathogen.
光代表了生物体评估其环境的普遍信息来源。针对三种分离株测定了光对菌落生长和产孢的影响。在红光下观察到这三种分离株的菌丝体生长速率最高，其次是绿光、日光或黑暗环境。然而，在黑暗和红光条件下观察到产孢水平降低，但在日光、蓝光和绿光下，随着光照时间延长，产孢有所增强。已鉴定出假定的蓝光（白领蛋白和隐花色素）、绿光（视蛋白）和红光（光敏色素）光感受器，以及与光反应相关的 velvet 蛋白调控家族。发现了每种光感受器的独特直系同源物，并且它们各自的结构域结构高度保守。在日光或特定颜色光下，以及在时间进程光照条件下，对未发现的基因集进行了转录分析，发现光依赖性触发了 、 、 和 的基因表达，并且颜色光作为 、 、 和 表达的正诱导剂。 与其他光响应真菌具有一组高度保守的光感受器。我们的表型分析以及这种光感应机制的存在表明，存在专门用于调节这种病原体发育和传播的转录调控系统。
