König Emilia, Sali Virpi, Heponiemi Paulina, Salminen Seppo, Valros Anna, Junnikkala Sami, Heinonen Mari
Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, 04920 Saarentaus, Finland.
Functional Foods Forum, Faculty of Medicine, University of Turku, 20014 Turku, Finland.
Animals (Basel). 2021 Jan 7;11(1):113. doi: 10.3390/ani11010113.
We studied the fecal lactobacilli count and species diversity of growing pigs along with immune parameters associated with intestinal lactobacilli. Thirty pigs categorized as small (S, = 12) or large (L, = 18) at birth were followed from birth to slaughter in two commercial herds, H1 and H2. Herds differed in terms of their general management. We determined sow colostrum quality, colostrum intake, piglet serum immunoglobulins, and pig growth. We took individual fecal samples from pigs in the weaning and finishing units. We studied lactobacilli count and identified their diversity with 16S PCR. Total lactobacilli count increased in H1 and decreased in H2 between samplings. Lactobacilli species diversity was higher in H1 in both fecal sampling points, whereas diversity decreased over time in both herds. We identified altogether seven lactobacilli species with a maximum of five (one to five) species in one herd. However, a relatively large proportion of lactobacilli remained unidentified with the used sequencing technique. Small pigs had higher lactobacilli counts in both herds but the difference was significant only in H2 ( = 0.01). Colostrum quality was numerically better in H1 than in H2, where colostrum intake tended to be associated with total lactobacilli count ( = 0.05).
我们研究了生长猪的粪便乳酸杆菌计数和物种多样性，以及与肠道乳酸杆菌相关的免疫参数。在两个商业猪场H1和H2中，对30头出生时分为小体型（S，n = 12）或大体型（L，n = 18）的仔猪从出生跟踪至屠宰。两个猪场的总体管理方式不同。我们测定了母猪初乳质量、初乳摄入量、仔猪血清免疫球蛋白和仔猪生长情况。我们从断奶和育肥单元的仔猪中采集个体粪便样本。我们研究了乳酸杆菌计数，并通过16S PCR鉴定其多样性。在两次采样之间，H1猪场的总乳酸杆菌计数增加，而H2猪场的总乳酸杆菌计数减少。在两个粪便采样点，H1猪场的乳酸杆菌物种多样性均较高，而两个猪场的物种多样性均随时间下降。我们共鉴定出7种乳酸杆菌，每个猪场最多有5种（1至5种）。然而，使用的测序技术仍无法鉴定出相对较大比例的乳酸杆菌。在两个猪场中，小体型仔猪的乳酸杆菌计数均较高，但仅在H2猪场差异显著（P = 0.01）。H1猪场的初乳质量在数值上优于H2猪场，在H2猪场初乳摄入量往往与总乳酸杆菌计数相关（P = 0.05）。
