The Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, No. 40 of Nongke South Road, Hefei, 230031, Anhui, China.
Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Anhui Academy of Agriculture Science, Hefei, 230031, China.
Probiotics Antimicrob Proteins. 2020 Mar;12(1):194-203. doi: 10.1007/s12602-019-9514-3.
Supplementing suckling piglets with Lactobacillus reuteri isolated from a homologous source improves L. reuteri colonization number in the gastrointestinal tract, which can have health benefits. This study investigated dietary L. reuteri supplementation on the growth and health-including immune status-of piglets, as well as its colonization. A total of 60 sows with similar parity and body weight were allocated into one of three groups after secretion (n = 20 each, with 10 neonatal piglets of each): untreated control, L. reuteri supplementation, and antibiotic treatment. The experimental duration was 28 days, from birth of piglets to their group transferred. For the first 7 days after birth, all neonatal piglets were fed by sows. Piglets in the L. reuteri supplementation group were administered with 1.0 ml L. reuteri fermentation broth containing 5.0 × 10 CFU. From 7 to 28 days, piglets were given basal feed (control), basal feed supplemented with L. reuteri (1.0 × 10 CFU/g), or aureomycin (150 mg/kg). L. reuteri colonization in the distal jejunum and ileum was increased in piglets in the L. reuteri-supplemented as compared to the control group after 28 days, as determined by fluorescence in situ hybridization and real-time PCR analysis. Total Lactobacillus and Bifidobacterium counts in the cecum were higher whereas total aerobic bacteria (Escherichia coli and Staphylococcus) counts were lower in the L. reuteri as compared to the control group. L. reuteri supplementation also improved body antioxidant status and immune function relative to control animals. Strain-specific L. reuteri administered to piglets colonizes the intestinal mucosa and improves cecal microbiota profile and whole-body antioxidant and immune status, leading to better growth and lower morbidity and mortality rates.
给哺乳仔猪补充源自同源的罗伊氏乳杆菌可提高其胃肠道内罗伊氏乳杆菌的定植数量，从而带来健康益处。本研究调查了日粮中补充罗伊氏乳杆菌对仔猪生长和健康（包括免疫状态）及其定植的影响。60 头具有相似胎次和体重的母猪在分娩后（每组 20 头，每头母猪 10 头仔猪）被分为三组之一：未处理对照组、罗伊氏乳杆菌补充组和抗生素处理组。实验持续 28 天，从仔猪出生到转入组。出生后前 7 天，所有新生仔猪都由母猪喂养。罗伊氏乳杆菌补充组仔猪灌喂 1.0ml 含有 5.0×10CFU 的罗伊氏乳杆菌发酵液。从第 7 天到第 28 天，仔猪给予基础饲料（对照组）、基础饲料添加罗伊氏乳杆菌（1.0×10CFU/g）或金霉素（150mg/kg）。通过荧光原位杂交和实时 PCR 分析，28 天后，与对照组相比，补充罗伊氏乳杆菌的仔猪在空肠和回肠远端的罗伊氏乳杆菌定植增加。与对照组相比，补充罗伊氏乳杆菌组的盲肠中总乳酸杆菌和双歧杆菌数量较高，而总需氧菌（大肠杆菌和葡萄球菌）数量较低。与对照组相比，补充罗伊氏乳杆菌还改善了仔猪的体抗氧化状态和免疫功能。特异性罗伊氏乳杆菌定植于仔猪肠道黏膜，并改善了盲肠微生物群谱和全身抗氧化和免疫状态，从而实现更好的生长和更低的发病率和死亡率。
