Department of Physiology, MGill University, Montreal, Canada.
Department of Physics and Astronomy, University of Exeter, Exeter, United Kingdom.
Elife. 2021 Jan 12;10:e56426. doi: 10.7554/eLife.56426.
We introduce a random-access parallel (RAP) imaging modality that uses a novel design inspired by a Newtonian telescope to image multiple spatially separated samples without moving parts or robotics. This scheme enables near-simultaneous image capture of multiple petri dishes and random-access imaging with sub-millisecond switching times at the full resolution of the camera. This enables the RAP system to capture long-duration records from different samples in parallel, which is not possible using conventional automated microscopes. The system is demonstrated by continuously imaging multiple cardiac monolayer and preparations.
我们介绍了一种随机访问并行(RAP)成像模式,该模式采用受牛顿望远镜启发的新颖设计,无需移动部件或机器人即可对多个空间分离的样本进行成像。该方案可实现多个培养皿的近同时图像采集,并具有亚毫秒级的切换时间,可达到相机的全分辨率。这使得 RAP 系统能够并行捕获来自不同样本的长时间记录,而这是使用传统自动化显微镜无法实现的。该系统通过连续成像多个心脏单层和 制剂得到了验证。