基于整合生物信息学分析鉴定透明细胞肾细胞癌的生物标志物并构建 microRNA-mRNA 调控网络。
Identification of biomarkers and construction of a microRNA‑mRNA regulatory network for clear cell renal cell carcinoma using integrated bioinformatics analysis.
机构信息
Department of Nephrology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
出版信息
PLoS One. 2021 Jan 12;16(1):e0244394. doi: 10.1371/journal.pone.0244394. eCollection 2021.
With the recent research development, the importance of microRNAs (miRNAs) in renal clear cell carcinoma (CCRCC) has become widely known. The purpose of this study is to screen out the potential biomarkers of renal clear cell carcinoma (CCRCC) by microarray analysis. The miRNA chip (GSE16441) and mRNA chip (GSE66270) related to CCRCC were downloaded from the Gene Expression Omnibus (GEO) database. After data filtering and pretreating, R platform and a series of analysis tools (funrich3.1.3, string, Cytoscape_ 3.2.1, David, etc.) were used to analyze chip data and identify the specific and highly sensitive biomarkers. Finally, by constructing the miRNA -mRNA interaction network, it was determined that five miRNAs (hsa-mir-199a-5p, hsa-mir-199b-5p, hsa-mir-532-3p and hsa-mir-429) and two key genes (ETS1 and hapln1) are significantly related to the overall survival rate of patients.
随着最近的研究进展，微小 RNA（miRNA）在肾透明细胞癌（CCRCC）中的重要性已被广泛认识。本研究旨在通过微阵列分析筛选出肾透明细胞癌（CCRCC）的潜在生物标志物。从基因表达综合数据库（GEO）下载了与 CCRCC 相关的 miRNA 芯片（GSE16441）和 mRNA 芯片（GSE66270）。经过数据过滤和预处理后，使用 R 平台和一系列分析工具（funrich3.1.3、string、Cytoscape_3.2.1、David 等）分析芯片数据并识别特定且高度敏感的生物标志物。最后，通过构建 miRNA-mRNA 相互作用网络，确定了五个 miRNA（hsa-mir-199a-5p、hsa-mir-199b-5p、hsa-mir-532-3p 和 hsa-mir-429）和两个关键基因（ETS1 和 hapln1）与患者的总生存率显著相关。
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