大鼠臂丛神经撕脱伤致神经病理性疼痛的潜在生物标志物的基因芯片分析。
Microarray analysis of potential biomarkers of brachial plexus avulsion caused neuropathic pain in male rat.
机构信息
Department of Pediatric Surgery, Affiliated Ruijin Hospital, Shanghai Jiao Tong University Medical School, Shanghai, People's Republic of China.
Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
出版信息
BMC Neurosci. 2022 May 26;23(1):31. doi: 10.1186/s12868-022-00717-9.
The present study aimed to investigate the expression of mRNA in the brachial plexus avulsion neuropathic pain model and analyze biological functions. Microarray mRNA assay and reverse transcriptase quantitative polymerase chain reaction (RT-PCR) were conducted. The whole blood was collected from two groups for Microarray mRNA analysis. The predicted mRNA targets were studied by gene ontology analysis and pathway analysis. We identified 3 targeted mRNAs, including PIK3CB, HRAS, and JUN. The results showed that PIK3CB, HRAS, and JUN gene expression was increased in the control group but decreased in the neuropathic pain group. These findings indicate that certain genes may be important biomarkers for the potential targets for the prevention and treatment of brachial plexus avulsion caused neuropathic pain.
本研究旨在探讨臂丛神经撕脱性神经病模型中 mRNA 的表达，并分析其生物学功能。采用微阵列 mRNA 检测和逆转录定量聚合酶链反应（RT-PCR）进行检测。收集两组全血进行微阵列 mRNA 分析。通过基因本体分析和通路分析研究预测的 mRNA 靶标。我们鉴定了 3 个靶向 mRNA，包括 PIK3CB、HRAS 和 JUN。结果表明，PIK3CB、HRAS 和 JUN 基因表达在对照组中增加，而在神经病理性疼痛组中降低。这些发现表明，某些基因可能是预防和治疗臂丛神经撕脱引起的神经病理性疼痛的潜在靶点的重要生物标志物。
Zotero 插件