Sun Lingmei, Liao Kai, Wang Dayong
Medical School, Southeast University, Nanjing 210009, China.
Medical School, Southeast University, Nanjing 210009, China.
Sci Total Environ. 2021 May 10;768:144362. doi: 10.1016/j.scitotenv.2020.144362. Epub 2020 Dec 29.
Certain modifications can aggravate the toxicity of nanoplastics. However, the influence of surface amino modification on transgenerational impairment induced by nanoplastics remains largely unclear. Pristine nanopolystyrene (NPS) and amino modified NPS (NPS-NH) were used to determine their transgenerational toxicity in Caenorhabditis elegans. Exposure to 100 μg/L pristine NPS in parents (P0) cause a decrease in reproductive capacity in the F1-F3 generations and the damage on gonad development in the F1-F2 generations. In contrast, exposure to 10 μg/L NPS-NH caused toxicity on reproductive capacity and gonad development in the F1 generation. The toxic effects of NPS-NH on reproductive capacity and gonad development in the F1-F3 generations were more severe than those of pristine NPS. Moreover, amino modification could increase transgenerational toxicity of NPS in inducing apoptosis of germline and in affecting expressions of ced-1, ced-4, and ced-9. Our data demonstrate that surface modification of NPS with amino groups enhances transgenerational reproductive toxicity of NPS in C. elegans.
某些修饰会加剧纳米塑料的毒性。然而，表面氨基修饰对纳米塑料诱导的跨代损伤的影响在很大程度上仍不清楚。使用原始纳米聚苯乙烯（NPS）和氨基修饰的NPS（NPS-NH）来确定它们在秀丽隐杆线虫中的跨代毒性。亲代（P0）暴露于100μg/L的原始NPS会导致F1-F3代的生殖能力下降以及F1-F2代的性腺发育受损。相比之下，暴露于10μg/L的NPS-NH会对F1代的生殖能力和性腺发育产生毒性。NPS-NH对F1-F3代生殖能力和性腺发育的毒性作用比原始NPS更严重。此外，氨基修饰可增加NPS在诱导生殖细胞凋亡以及影响ced-1、ced-4和ced-9表达方面的跨代毒性。我们的数据表明，用氨基对NPS进行表面修饰会增强NPS在秀丽隐杆线虫中的跨代生殖毒性。
