State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
College of Life Science, Yangtze University, Jingzhou, Hubei 434023, People's Republic of China.
Anal Chem. 2021 Feb 2;93(4):2090-2096. doi: 10.1021/acs.analchem.0c03836. Epub 2021 Jan 13.
The sensitive detection of coronavirus is of vital importance for the prevention of its rapid spread. Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe diarrhea and death in neonatal piglets. In this work, a novel PCN-224-based electrochemiluminescence (ECL) system was constructed for PEDV detection with high sensitivity. We found that PCN-224 can be employed as an ECL reporter with a strong signal because of its zirconium-based organic porous frame nanomaterial with a large specific surface area and stable structure. TiO nanoparticles were used as an accelerator for the first time to promote the reduction of coreactant potassium peroxydisulfate on the cathode; thus, the initial ECL signal of PCN-224 was significantly amplified. In the presence of PEDV, the ECL signal decreased due to the block effect to electron transfer. As a result, the novel "signal off" biosensor achieved a sensitive detection of PEDV ranging from 1 pg/mL to 10 ng/mL, with a detection limit of 0.4 pg/mL (S/N = 3). Importantly, the PCN-224 nanomaterial enriched the ECL system in biological analysis, and the proposed strategy provided a new route for coronavirus detection.
冠状病毒的灵敏检测对于其快速传播的预防至关重要。猪流行性腹泻病毒（PEDV）是一种高度传染性的冠状病毒，可导致新生仔猪严重腹泻和死亡。在这项工作中，构建了一种基于 PCN-224 的新型电致化学发光（ECL）系统，用于PEDV 的高灵敏度检测。我们发现，由于其具有大比表面积和稳定结构的基于锆的有机多孔框架纳米材料，PCN-224 可用作 ECL 报告器，具有很强的信号。首次将 TiO2 纳米颗粒用作加速剂，以促进阴极上的核反应物过硫酸钾的还原；因此，PCN-224 的初始 ECL 信号得到了显著放大。在存在 PEDV 的情况下，由于电子转移的阻断效应，ECL 信号降低。结果，新型“信号关闭”生物传感器实现了对 PEDV 的灵敏检测，检测范围为 1 pg/mL 至 10 ng/mL，检测限为 0.4 pg/mL（S/N = 3）。重要的是，PCN-224 纳米材料丰富了生物分析中的 ECL 系统，所提出的策略为冠状病毒检测提供了新途径。
