Nam Youn Hee, Jeong Seo Yule, Kim Yun Hee, Rodriguez Isabel, Nuankaew Wanlapa, Bhawal Ujjal K, Hong Bin Na, Kang Tong Ho
Department of Oriental Medicine Biotechnology, College of Life Sciences and Graduate School of Biotechnology, Kyung Hee University, Gyeonggi, Republic of Korea.
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
J Ginseng Res. 2021 Jan;45(1):183-190. doi: 10.1016/j.jgr.2020.09.003. Epub 2020 Sep 16.
The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte () gene is related to circadian rhythm. To our knowledge, there are no reports of the relationship between dec gene expression and KRG effect. Therefore, we treated gene knockout (KO) aging mice with KRG to study anti-aging related effects and possible mechanisms.
We evaluated KRG and expression of genes in an ototoxicity model. genes expression in livers of aging mice was further analyzed. Then, we assessed the effects of DEC KO on hearing function in mice by ABR. Finally, we performed DNA microarray to identify KRG-related gene expression changes in mouse liver and assessed the results using KEGG analysis.
KRG decreased the expression of genes in ototoxicity model, which may contribute to its anti-aging efficacy. Moreover, KRG suppressed genes expression in liver of wild type indicating inhibition of senescence. ABR test indicated that KRG improved auditory function in aging mouse, demonstrating KRG efficacy on aging related diseases.
Finally, in KEGG analysis of 238 genes that were activated and 158 that were inhibited by KRG in DEC KO mice, activated genes were involved in proliferation signaling, mineral absorption, and PPAR signaling whereas the inhibited genes were involved in arachidonic acid metabolism and peroxisomes. Our data indicate that inhibition of senescence-related genes may explain the anti-aging efficacy of KRG.
昼夜节律是控制睡眠 - 觉醒周期、新陈代谢、认知及身体其他多种过程的内部时钟，其紊乱与衰老相关。分化胚胎软骨细胞（）基因与昼夜节律有关。据我们所知，尚无关于dec基因表达与红参（KRG）作用关系的报道。因此，我们用红参处理基因敲除（KO）衰老小鼠，以研究其抗衰老相关作用及可能机制。
我们在耳毒性模型中评估了红参与基因的表达。进一步分析衰老小鼠肝脏中基因的表达。然后，我们通过听性脑干反应（ABR）评估DEC基因敲除对小鼠听力功能的影响。最后，我们进行DNA微阵列分析以鉴定小鼠肝脏中与红参相关的基因表达变化，并使用京都基因与基因组百科全书（KEGG）分析评估结果。
在耳毒性模型中，红参降低了基因的表达，这可能有助于其抗衰老功效。此外, 红参抑制野生型小鼠肝脏中基因的表达，表明其对衰老有抑制作用。ABR测试表明红参改善了衰老小鼠的听觉功能，证明了红参对衰老相关疾病的功效。
最后，在KEGG分析中，红参在DEC基因敲除小鼠中激活了238个基因，抑制了158个基因，激活的基因参与增殖信号传导、矿物质吸收和过氧化物酶体增殖物激活受体（PPAR）信号传导，而抑制的基因参与花生四烯酸代谢和过氧化物酶体。我们的数据表明，抑制与衰老相关的基因可能解释了红参的抗衰老功效。
