巴基斯坦高血压孕妇中前列腺素基因的遗传多态性
Genetic polymorphism of the prostasin gene in hypertensive pregnant Pakistani females.
作者信息
Ejaz Saima, Ali Anwar, Riffat Sumaira, Mahmood Atif, Azim Kamran
机构信息
Saima Ejaz Ph.D. Scholar, Department of Physiology, University of Karachi, Pakistan.
Anwar Ali Assistant Professor, Department of Physiology, University of Karachi, Pakistan.
出版信息
Pak J Med Sci. 2021 Jan-Feb;37(1):109-113. doi: 10.12669/pjms.37.1.3666.
OBJECTIVE
The study was performed to investigate the association of hypertension in pregnancy with prostasin gene polymorphism in Pakistani females.
METHODS
This case-control study was performed at University of Karachi, Pakistan from April 2018 to May 2019. A total of 160 females, including 90 hypertensives and 70 healthy pregnant females, were recruited by purposive sampling after obtaining informed written consent. Genotyping was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
RESULTS
The frequencies of the TC and CC genotypes were higher in hypertensive pregnant females compared to healthy controls. A significant difference was evident for CC (P=0.012) genotype; however, no significant difference was observed for TC (P=0.49) and TT genotypes (P=0.06) between control and hypertensive groups. The adjusted odds ratio for CC genotype was 6.2 (P=0.025) and 1.48 (P=0.44) for TC genotype compared to the TT genotype. There was a significantly higher prevalence of the C allele of the prostasin gene at rs12597511 in the hypertensive group, suggesting that this allele is a risk factor for hypertension and cardiovascular diseases.
CONCLUSION
C allele at rs12597511 of prostasin gene demonstrate as a risk factor for having hypertension in pregnancy.
目的
本研究旨在调查巴基斯坦女性妊娠期高血压与前列腺素酶基因多态性之间的关联。
方法
本病例对照研究于2018年4月至2019年5月在巴基斯坦卡拉奇大学进行。在获得知情书面同意后，通过目的抽样法招募了160名女性，其中包括90名高血压患者和70名健康孕妇。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）进行基因分型。
结果
与健康对照组相比，妊娠期高血压女性中TC和CC基因型的频率更高。CC基因型（P=0.012）存在显著差异；然而，对照组和高血压组之间的TC基因型（P=0.49）和TT基因型（P=0.06）未观察到显著差异。与TT基因型相比，CC基因型的调整优势比为6.2（P=0.025），TC基因型为1.48（P=0.44）。高血压组中rs12597511处前列腺素酶基因C等位基因的患病率显著更高，表明该等位基因是高血压和心血管疾病的危险因素。
结论
前列腺素酶基因rs12597511处的C等位基因被证明是妊娠期高血压的危险因素。
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