Ye Yongheng, Peng Yue, He Peiheng, Zhang Qinqin, Xu Dongliang
Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, China.
Department of Otorhinolaryngology Head and Neck Surgery, Zhuhai People's Hospital, Zhuhai, Guangdong Province, 519000, China.
J Orthop Translat. 2020 Feb 21;26:54-59. doi: 10.1016/j.jot.2020.01.008. eCollection 2021 Jan.
Urinary microRNAs (miRNAs) have shown great diagnostic and prognostic values for multiple diseases. The profile of urinary miRNAs in patients with idiopathic osteonecrosis of femoral head (ONFH) is currently unclear.
We first randomly chose ten patients with each Association Research Circulation Osseous (ARCO) stage (I, II, III and IV) and ten healthy participants from the entire cohorts for initial screening. The miRNA polymerase chain reaction (PCR) array was then performed to identify the differentially abundant miRNAs in urine of these participants. We then verified the findings in the entire cohort. Clinical features including age, gender, bone mass index (BMI), lesion size and stages were recorded. We then analysed the association between the level of urinary miRNAs and clinical features.
Our data indicated that there were 13 differentially abundant miRNAs among all groups. Urinary miR-150 demonstrated the highest diagnostic value among all candidates. Urinary miR-185 and miR-133a increased by ARCO staging. The levels of urinary miR-4824 abruptly decreased after femoral head collapse (ARCO stage III and IV). Urinary miR-144 was the only marker that correlated with lesion size.
The levels of urinary miRNAs are valuable biomarkers for idiopathic ONFH. Given the noninvasive nature of this test, it is potentially useful for diagnosis and monitoring of idiopathic ONFH progression.
This article gives novel methods for ONFH diagnosis and progression monitoring in a convenient and non-invasive way.
尿微小RNA（miRNA）已显示出对多种疾病具有巨大的诊断和预后价值。目前，股骨头缺血性坏死（ONFH）患者尿miRNA的特征尚不清楚。
我们首先从整个队列中随机选择10例处于骨循环研究协会（ARCO）各期（I、II、III和IV期）的患者以及10名健康参与者进行初步筛查。然后进行miRNA聚合酶链反应（PCR）芯片检测，以鉴定这些参与者尿液中丰度有差异的miRNA。随后我们在整个队列中验证了这些发现。记录包括年龄、性别、骨质量指数（BMI）、病变大小和分期等临床特征。然后我们分析了尿miRNA水平与临床特征之间的关联。
我们的数据表明，所有组之间有13种丰度有差异的miRNA。尿miR-150在所有候选miRNA中显示出最高的诊断价值。尿miR-185和miR-133a随ARCO分期增加。股骨头塌陷后（ARCO III期和IV期），尿miR-4824水平急剧下降。尿miR-144是唯一与病变大小相关的标志物。
尿miRNA水平是特发性ONFH的有价值生物标志物。鉴于该检测的非侵入性，它可能对特发性ONFH的诊断和病情监测有用。
本文以方便且非侵入性的方式提供了ONFH诊断和病情监测的新方法。
