Invasive Technology Department, Jiangsu Cancer Hospital, Nanjing, Jiangsu, China.
Rectal Surgery Department, Jiangsu Cancer Hospital, Nanjing, Jiangsu, China.
Braz J Med Biol Res. 2021 Jan 8;54(2):e10394. doi: 10.1590/1414-431X202010394. eCollection 2021.
MicroRNAs (miRNAs) have been indicated to be frequently dysregulated in various cancers and promising biomarkers for colon cancer. The present study aimed to assess the prognostic significance and biological function of miR-1273a in colon cancer. The expression levels of miR-1273a was estimated using quantitative real-time polymerase chain reaction. Kaplan-Meier survival curves and Cox regression analysis were used to evaluate the prognostic value of miR-1273a in patients of colon cancer. The effects of miR-1273a on cell proliferation, migration, and invasion were investigated by cell experiments. The expression of miR-1273a was downregulated in colon cancer tissues and tumor cell lines compared with the normal controls (all P<0.001). The aberrant expression of miR-1273a was associated with vascular invasion (P=0.005), differentiation (P=0.023), lymph node metastasis (P=0.021), and TNM stage (P=0.004). The patients with low miR-1273a expression had low overall survival compared with the patients with high miR-1273a expression (log-rank P=0.002). miR-1273a was detected to be an independent prognostic biomarker for patients. Furthermore, the results of cell experiments revealed that miR-1273a downregulation promoted, while miR-1273a upregulation suppressed the cell proliferation, migration, and invasion. In conclusion, all data indicated that a downregulated expression of miR-1273a predicted poor prognosis for colon cancer and enhanced tumor cell proliferation, migration, and invasion. Thus, we suggest that methods to promote miR-1273a expression may serve as novel therapeutic strategies in colon cancer.
微小 RNA（miRNAs）在各种癌症中经常失调，是结肠癌有前途的生物标志物。本研究旨在评估 miR-1273a 在结肠癌中的预后意义和生物学功能。使用定量实时聚合酶链反应估计 miR-1273a 的表达水平。Kaplan-Meier 生存曲线和 Cox 回归分析用于评估 miR-1273a 对结肠癌患者的预后价值。通过细胞实验研究 miR-1273a 对细胞增殖、迁移和侵袭的影响。与正常对照组相比，结肠癌组织和肿瘤细胞系中 miR-1273a 的表达下调（均 P<0.001）。miR-1273a 的异常表达与血管侵犯（P=0.005）、分化（P=0.023）、淋巴结转移（P=0.021）和 TNM 分期（P=0.004）相关。miR-1273a 低表达的患者总生存期低于 miR-1273a 高表达的患者（对数秩 P=0.002）。miR-1273a 被检测为患者的独立预后生物标志物。此外，细胞实验结果表明，miR-1273a 下调促进，而 miR-1273a 上调抑制细胞增殖、迁移和侵袭。总之，所有数据表明，miR-1273a 的下调表达预示着结肠癌预后不良，并增强了肿瘤细胞的增殖、迁移和侵袭。因此，我们建议促进 miR-1273a 表达的方法可能成为结肠癌的新治疗策略。
