Second Department of Radiotherapy, Yantai Hill Hospital, Yantai, 264003, Shandong, China.
Department of Gastroenterology, Weifang People's Hospital, Weifang, 261000, Shandong, China.
Clin Exp Med. 2022 May;22(2):193-200. doi: 10.1007/s10238-021-00753-5. Epub 2021 Sep 1.
Colon cancer is one of most common cancers. The progression of various cancers is driven by miRNA-570. The role of miRNA-570 in the progression of colon cancer remains unclear. We aimed to investigate the clinical function of miR-570 and its impact on colon cancer cells. We evaluated the expression of miR-570 in colon cancer cells and analyzed its influence on the various clinical parameters. The Kaplan-Meier curve was plotted to understand the clinical role of miR-570. Cox regression analysis was performed to predict the prognostic factors in colon cancer. The Cell Counting Kit-8 was used to investigate the effect of miR-570 on cell proliferation. The transwell migration assay was performed to quantify cell migration and invasion. The quantitative real-time polymerase chain reaction technique was used to analyze the sample system. The results revealed that the level of miR-570 expression in colon cancer tissues and cell lines was low. The abnormal expression of miR-570 was associated with tumor size, extent of differentiation, lymph node metastasis, and tumor-node-metastasis stages. Downregulation of miR-570 indicated poor overall survival (OS), poor relapse-free survival, and unfavorable cancer-specific survival (CSS) rates in patients with colon cancer. The results from Cox regression analysis revealed that miR-570 expression could be used as an independent prognostic biomarker for OS and CSS in colon cancer. Overexpression of miR-570 can potentially result in the inhibition of cell proliferation, migration, and invasion. The results proved that miR-570 could potentially function as a tumor suppressor and a potential prognostic factor in patients with colon cancer.
结肠癌是最常见的癌症之一。各种癌症的进展都受 miRNA-570 的驱动。miRNA-570 在结肠癌进展中的作用尚不清楚。我们旨在研究 miR-570 的临床功能及其对结肠癌细胞的影响。我们评估了结肠癌细胞中 miR-570 的表达，并分析了其对各种临床参数的影响。绘制 Kaplan-Meier 曲线以了解 miR-570 的临床作用。进行 Cox 回归分析以预测结肠癌的预后因素。使用细胞计数试剂盒-8 来研究 miR-570 对细胞增殖的影响。进行 Transwell 迁移测定以量化细胞迁移和侵袭。使用定量实时聚合酶链反应技术分析样本系统。结果表明，结肠癌组织和细胞系中 miR-570 的表达水平较低。miR-570 的异常表达与肿瘤大小、分化程度、淋巴结转移和肿瘤-淋巴结-转移分期有关。miR-570 的下调表明结肠癌患者的总生存率（OS）、无复发生存率和不利的癌症特异性生存率（CSS）较差。Cox 回归分析的结果表明，miR-570 的表达可作为结肠癌 OS 和 CSS 的独立预后生物标志物。miR-570 的过表达可能导致细胞增殖、迁移和侵袭的抑制。结果证明，miR-570 可能在结肠癌患者中作为肿瘤抑制因子和潜在的预后因素发挥作用。
