Yang Daye, Zhang Dewei
Department of 3rd General Surgery, The Fourth Affiliated Hospital of China Medical University, Chongshan East Road in Huanggu District, Shenyang, Liaoning, 11032, China.
Clin Exp Med. 2021 Nov;21(4):563-571. doi: 10.1007/s10238-021-00706-y. Epub 2021 Apr 10.
MiR-1253 has been reported to play vital roles in a variety of cancers. However, its function in the progression and prognosis of colon cancer remains unclear. Here, the clinical significance and biological function of miR-1253 in the development of colon cancer was investigated. The expression of miR-1253 was identified in colon cancer tissues and cell lines by qRT-PCR. The prognostic value of miR-1253 was evaluated by Kaplan-Meier and Cox regression analysis. The roles of miR-1253 in regulating cell proliferation, migration, and invasion of colon cancer were evaluated in vitro by CCK8 and Transwell assay. It was found that miR-1253 was significantly downregulated in colon cancer tissues and cell lines (P < 0.001). The downregulation of miR-1253 was associated with the TNM stage (P = 0.007) and lymph node metastasis (P = 0.037) of patients. MiR-1253 (HR factor = 2.564, 95% CI = 1.077-6.107, P = 0.033) and TNM stage (HR factor = 2.899, 95% CI = 1.024-8.205, P = 0.045) were identified as independent factors that can predict the prognosis of patients. Functionally, miR-1253 acts as a tumor suppressor that inhibited cell proliferation, migration, and invasion of colon cancer through targeting EZH2. MiR-1253 was identified as a prognostic biomarker and a tumor suppressor of colon cancer.
据报道，miR-1253在多种癌症中发挥着重要作用。然而，其在结肠癌进展和预后中的作用仍不清楚。在此，研究了miR-1253在结肠癌发生发展中的临床意义和生物学功能。通过qRT-PCR检测结肠癌组织和细胞系中miR-1253的表达。采用Kaplan-Meier法和Cox回归分析评估miR-1253的预后价值。通过CCK8和Transwell实验在体外评估miR-1253对结肠癌细胞增殖、迁移和侵袭的影响。结果发现，miR-1253在结肠癌组织和细胞系中显著下调（P < 0.001）。miR-1253的下调与患者的TNM分期（P = 0.007）和淋巴结转移（P = 0.037）相关。miR-1253（HR因子 = 2.564，95% CI = 1.077 - 6.107，P = 0.033）和TNM分期（HR因子 = 2.899，95% CI = 1.024 - 8.205，P = 0.045）被确定为可预测患者预后的独立因素。在功能上，miR-1253作为一种肿瘤抑制因子，通过靶向EZH2抑制结肠癌细胞的增殖、迁移和侵袭。miR-1253被确定为结肠癌的预后生物标志物和肿瘤抑制因子。
